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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E_inD)Luc

Plasmid

#200109

Purpose

Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element in front of EGR1 promoter…

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiDP1.0

Plasmid

#171094

Purpose

lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

Depositor

Dario Palmieri

Article

Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

Insert

OsTIR1-mEmerald-P2A-miniAID-mCherry2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4 CoV-2 nsp1 3xFLAG

Plasmid

#176057

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1163

Plasmid

#111099

Purpose

Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR LAT/Zap70 iLID-Drop

Plasmid

#171038

Purpose

Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

LAT (LAT Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL8A-GFP

Plasmid

#67403

Purpose

Mammalian expression of hArl8 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-OB-Linker-eGFP

Plasmid

#176068

Purpose

EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

OB

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo WT nsp1

Plasmid

#175412

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1 (ORF1ab CoV2)

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Macro-Linker-eGFP

Plasmid

#176067

Purpose

EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Macro

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-FHA-Linker-eGFP

Plasmid

#176065

Purpose

EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

FHA

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-WWE-Linker-eGFP

Plasmid

#176072

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

WWE

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL4C-GFP

Plasmid

#67402

Purpose

Mammalian expression of hArl4c with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLV-Hygro-EF1A-PBZC-Linker-eGFP

Plasmid

#176070

Purpose

EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBZ-C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-PBM-Linker-eGFP

Plasmid

#176069

Purpose

EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBM

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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