We narrowed to 13,644 results for: NTS

1. FusionRed-Lifeact-7

   Plasmid

   #54778

   Purpose

   Localization: Actin, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Type

   Empty backbone

   Use

   Tags

   FusionRed-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pBac{3XP3::EYFP,attP}

   Plasmid

   #86860

   Purpose

   Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Insert

   attP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2T-CMV-AIDmax-BlastR

   Plasmid

   #152994

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   AID-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p2T-CMV-ABEmaxCP1041-BlastR

   Plasmid

   #152990

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   ABE7.10-nSpCas9 CP1041
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p2T-CMV-BE4maxCP1028-BlastR

   Plasmid

   #152993

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9 CP1028-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRII-1.1-1.10a

   Plasmid

   #69589

   Purpose

   OT-II TCR a chain cDNA in pCRII

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR alpha clone 1.1 (Tcra Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBJ3

   Plasmid

   #167132

   Purpose

   A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Frr promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2T-CMV-CDAmax-BlastR

   Plasmid

   #152995

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   CDA-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP

   Plasmid

   #172001

   Purpose

   Expression for LIBRON constructs

   Depositor

   Thomas Wandless

   Article

   Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

   Insert

   FKBP DD (L106P)-UbK0G75C
   

   Use

   Retroviral

   Tags

   sfGFP

   Expression

   Mutation

   change all lysin residues of ubiquitin to Arg, G7…

   Promoter

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMOD_A0101

    Plasmid

    #90998

    Purpose

    Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRANS_221

    Plasmid

    #91115

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JD631

    Plasmid

    #160399

    Purpose

    Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 190_SMAD4 in pmiRGlo

    Plasmid

    #78128

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5_tAKARβ

    Plasmid

    #119914

    Purpose

    Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARβ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Use

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    copFnull

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

    Plasmid

    #49772

    Purpose

    Level 2 construct

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    sgRNA_PDS2-Cas9-sgRNA_PDS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CAG-DIO-mTurquoise2

    Plasmid

    #104059

    Purpose

    AAV expression of Cre-dependent reporter mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211840

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

    Plasmid

    #200637

    Purpose

    Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    Vif
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T27stop

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LTR5HS S. pyogenes scaffold CARGO array

    Plasmid

    #191316

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR TDP43 WT YFP

    Plasmid

    #27470

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ133-RZ-Cas12j2

    Plasmid

    #189784

    Purpose

    Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMOD_A0501

    Plasmid

    #91011

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pmCherry-T2A-moxMaple3

    Plasmid

    #120875

    Purpose

    Mammalian expression of mCherry and moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Inserts

    mCherry
    moxMaple3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ141D2.0

    Plasmid

    #99906

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRK5-FLAG-SZT2

    Plasmid

    #87034

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. xCas9 3.6

    Plasmid

    #108384

    Purpose

    Mammalian expression vector for xCas9 3.6

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. R57C10-Flp2

    Plasmid

    #63172

    Purpose

    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

40. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

    Plasmid

    #192651

    Purpose

    3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-10xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge