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pDSM-f3

Plasmid

#127722

Purpose

Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tadh1

Plasmid

#127723

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Pact1

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Ppre3-ITE-Teno1

Plasmid

#127724

Purpose

Yeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppre3

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tdpp1

Plasmid

#127725

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Pact1

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Phta1-ITE-Tvma2

Plasmid

#127726

Purpose

Yeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Expression

Yeast

Promoter

Phta1

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prps3

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Peft2-CAD-Tklura3

Plasmid

#127729

Purpose

Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peft2

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psmtef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pykt6

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptef1

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Ppgk1-PYC-Ttpi1

Plasmid

#127735

Purpose

Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppgk1

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Ppxr1-PYC-Tnat1

Plasmid

#127736

Purpose

Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppxr1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pzuo1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppfy1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Peno2-ACDH-Ttal1

Plasmid

#127743

Purpose

Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peno2

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Pvma6-ACDH-Trps14a

Plasmid

#127744

Purpose

Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pvma6

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Prpl28-ACDH-Tecm10

Plasmid

#127745

Purpose

Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prpl28

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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Tubb3gRNA-mCherry

Plasmid

#87111

Purpose

mouse Tubb3 target gRNA expression plasmid with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Mouse Tubb3 gRNA

Use

CRISPR

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_ABE_D3_empty

Plasmid

#203809

Purpose

Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

ABE_D3

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiR-report-MYB-mut

Plasmid

#141115

Purpose

Reporter plasmid of mutant MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Luciferase

Expression

Mammalian

Mutation

A to T mutations at m6A sites

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCK020

Plasmid

#105379

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor WUSCHEL (At3g11260; 2300bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pK-bes

Plasmid

#254055

Purpose

Suicide vector for allelic exchange with sucrose-based negative selection via sacB gene. Derived from pKNOCK-bla-ermGb and pCVD442.

Depositor

Juliane Bubeck-Wardenburg

Type

Empty backbone

Use

Allelic exchange in bacteroides

Promoter

rpoD (for sacB)

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB-U6-sgRNA-BFP-Puro

Plasmid

#229013

Purpose

Perturb Seq sgRNA vector with PiggyBac backbone

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-Flex-FlpO

Plasmid

#191202

Purpose

Cre dependent FlpO expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

FlpO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti ABE7.10-N-AIDmax

Plasmid

#157949

Purpose

Lentiviral expression of ABE7.10-N-AIDmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti ABE7.10-N-AIDmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gTBEv3

Plasmid

#220619

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

hUNG2-nSpCas9

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476)

Plasmid

#248216

Purpose

bpNLS-BxbI IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-bpNLS-BxbI-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1 attB pDonor (JO1529)

Plasmid

#248223

Purpose

Bxb1 attB pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Bxb1 attB

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_CAG(del)-FLEx(loxP)-mCherry(nuclear)-2A-N2cG

Plasmid

#248663

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear mCherry and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-mCherry

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hyA&C-BEmax

Plasmid

#196097

Purpose

Expresses hyA&C-Bemax

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

hyA&C-BEmax

Use

CRISPR

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
eA&C-BEmax

Plasmid

#196098

Purpose

Expresses eA&C-BEmax

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

eA&C-BEmax

Use

CRISPR

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvol-chKlacRS-WT

Plasmid

#232159

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-WT

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-3_Flag-YTHDF2

Plasmid

#238975

Purpose

Expresses YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2 (YTHDF2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-4

Plasmid

#228986

Purpose

For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4441 - 4480 of 6639 results

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We narrowed to 6,639 results for: WUS;