We narrowed to 11,193 results for: ENA

Showing: 2221 - 2240 of 11193 results

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDB179-Z1-PETase

Plasmid

#218703

Purpose

Encodes codon optimized Z1-PETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

Z1-PETase

Tags

10xHis

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-IRES-HO-1

Plasmid

#80345

Purpose

Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgRNA (empty)

Plasmid

#198330

Purpose

Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

U6-sgRNA(F+E) empty

Expression

Mammalian

Promoter

U6

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-HP1gamma

Plasmid

#17650

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 gamma (CBX3 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBHM2681

Plasmid

#241726

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDB179-DuraPETase

Plasmid

#218699

Purpose

Encodes codon optimized DuraPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DuraPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFP.E227

Plasmid

#138249

Purpose

Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

mCherry
Terminator
mTagBFP
Terminator
gfp
Terminator

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P17_up1691, P20_992, and iP16_3622

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-dAPEX2

Plasmid

#117173

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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pPIDNB

Plasmid

#169901

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Aivian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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TMEM63A-W472K-peGFP-N1

Plasmid

#228591

Purpose

Expresses mouse TMEM63A-W472K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

W472K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A0101

Plasmid

#90998

Purpose

Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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