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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,344 results for: Lif

Showing: 2221 - 2240 of 7344 results

  1. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  3. pCL1318

    Plasmid
    #217960
    Purpose
    Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    L63A, F66A, I71A
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217960-plasmid-map-sequence-id-430214

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  4. pCL1317

    Plasmid
    #217959
    Purpose
    Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between V65 and F66
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217959-plasmid-map-sequence-id-430213

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  5. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

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  6. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

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  17. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

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  19. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid
    #180653
    Purpose
    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CALPAIN-7
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    siRNA resistant to: GCACCCAUACCUUUACAUU
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180653-plasmid-map-sequence-id-358656

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Showing: 2221 - 2240 of 7344 results