We narrowed to 2,603 results for: ubiquitin

1. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

   Plasmid

   #225949

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C100A

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 CARD8 Ub-S297

   Plasmid

   #169965

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Ub

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Ubl4A K48R

   Plasmid

   #61809

   Purpose

   expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K48R

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJC6-3XHA-TMEM192

   Plasmid

   #104434

   Purpose

   Expresses 3XHA-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PADH1-TIR1-9myc-URA3

   Plasmid

   #207016

   Purpose

   Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   TIR1
   

   Use

   Tags

   9xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pADH1

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b_H6-Smt3-AesC-GFP

   Plasmid

   #234486

   Purpose

   Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   C-terminal Fragment of the Aes123 PolB1 Split Intein
   

   Use

   Tags

   Hexahistidine Tag, Superfolder Green Fluorescent …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ203 (pMDC32-Ubi1)

    Plasmid

    #86207

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1 (ZmUbi1)

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV3TAG8li-hRNF160-3FLAG

    Plasmid

    #159138

    Purpose

    human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    listerin E3 ubiquitin protein ligase 1 (LTN1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH-ABE8e

    Plasmid

    #200774

    Purpose

    For plant adenine base editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

    Insert

    nCas9(D10A)-TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNubWtXgate

    Plasmid

    #105079

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid

    #225947

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3E S17E S19E

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid

    #225948

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3A S17A S19A

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3e UbB polyA

    Plasmid

    #188702

    Purpose

    Gateway 3' element encoding the ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    UbB polyA (ubb Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA cyclin D1 HA T286A

    Plasmid

    #11182

    Depositor

    Bruce Zetter

    Article

    Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

    Insert

    cyclin D1 T286A (CCND1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T286A. Mutant that cannot be degraded via the u…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1180-HR-PUbEBFP2-NLS

    Plasmid

    #176682

    Purpose

    Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EBFP2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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