We narrowed to 14,065 results for: SHI

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NCOA4_RET

Plasmid

#205865

Purpose

Express mEGFP-tagged fusion protein, NCOA4_RET from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NCOA4_RET (NCOA4, RET Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

Plasmid

#231007

Purpose

Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

E4F1 aa220-242 (E4F1 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLI

Plasmid

#131228

Purpose

Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Coding sequence has been codon optimised for expr…

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 27, 2019

Availability

Academic Institutions and Nonprofits only

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