We narrowed to 9,643 results for: SUB

1. Nrp2.6-AP-His

   Plasmid

   #71977

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.6 (Nrp2 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sema3b(S)-AP-His

   Plasmid

   #72014

   Purpose

   Expresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3b (Sema3b Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nrp2.5-AP-His

   Plasmid

   #71976

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.5 (Nrp2 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Nrp2.4-AP-His

   Plasmid

   #71975

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.4 (Nrp2 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ntng1.j-AP-His

   Plasmid

   #71993

   Purpose

   Expresses the extracellular region of the Netrin G1, isoform j protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng1.j (Ntng1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30Xa-SBL1(160E,NoCys)

   Plasmid

   #66875

   Purpose

   Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
   

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   S160E; changed 4 (all) cysteines to serine

   Promoter

   T7lac

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SMR3B-bio-His

   Plasmid

   #52183

   Purpose

   Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SMR3B (SMR3B Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScalps_puro_mIL-2Rα ST

   Plasmid

   #59919

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain mutated

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Mutation

   changed sequence of the intracellular (C-terminal…

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSP64-hERG1a S620T

   Plasmid

   #53059

   Purpose

   Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Serine 620 to Threonine

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64-hERG1a S631A

    Plasmid

    #53058

    Purpose

    Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Serine 631 to Alanine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

    Plasmid

    #42185

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    G protein Gamma 3(F70A, F71A) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F70A, F71A

    Promoter

    cmv

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

    Plasmid

    #42188

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Gamma 3(K68A,K69A) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K68A,K69A

    Promoter

    cmv

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(+)mGAT1CFP45

    Plasmid

    #41676

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)mGAT1YFP45

    Plasmid

    #41675

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Comp AlphaPS3

    Plasmid

    #14071

    Depositor

    Richard Hynes

    Article

    Stark et al Development. 1997 Nov . 124(22):4583-94.

    Insert

    Drosphila Comp Alpha PS3 Integrin (scb Fly)
    

    Expression

    Bacterial

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nsp3 -EGFP

    Plasmid

    #165108

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp3 (ORF1ab SARS-COV-2)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nsp4-mCherry

    Plasmid

    #165132

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp4 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Orf3a-mCherry

    Plasmid

    #165138

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf3a (ORF3a codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWPI-LUC7L2-3xFLAG

    Plasmid

    #201641

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L2

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p3xFLAG-CMV14-GINIP

    Plasmid

    #204845

    Purpose

    Express 3x FLAG tagged-GINIP in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

    Insert

    PHF24 (KIAA1045 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 14, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Nsp13-mCherry

    Plasmid

    #165136

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

    Plasmid

    #134867

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR-CD80

    Plasmid

    #223603

    Purpose

    Lentiviral expression of human CD80 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pF4Ag NanoLuc (ATG-42)

    Plasmid

    #137777

    Purpose

    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

    Depositor

    Lance Encell

    Article

    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

    Insert

    NanoLuc (Nluc)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NanoLuc contains 16 amino acid changes compared t…

    Promoter

    T7 and CMV

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBABE HA Chk2 KD, dominant negative chk2

    Plasmid

    #41902

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D347A

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA 3.2/V5-DEST MTFR1L-V5

    Plasmid

    #69822

    Purpose

    Expresses MTFR1L-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Mitochondrial fission regulator 1-like (MTFR1L Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3-PAX3-FOXO1/FKHR

    Plasmid

    #115526

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

    Insert

    Pax3-FOXO1/FKHR (FOXO1, Pax3 Human, Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-hATG7wt

    Plasmid

    #87867

    Purpose

    Express a wild type human Atg7/Apg7 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 (ATG7 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. nAChR beta2 WT

    Plasmid

    #24272

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR beta2 WT (Chrnb2 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. nAChR alpha4 WT

    Plasmid

    #24271

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR alpha4 WT (Chrna4 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

36. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLCKO2

    Plasmid

    #125518

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Nsp6-mCherry

    Plasmid

    #165133

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp6 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge