We narrowed to 22,312 results for: CRISPR

1. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_KanR neo

   Plasmid

   #167861

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL690

   Plasmid

   #47753

   Purpose

   Expresses dCas9-VP64 fusion

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRU52

   Plasmid

   #167678

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUB4-2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9–mKate2ps–CgRNA

   Plasmid

   #64955

   Purpose

   Control gRNA (GTCAAGGCACTCTTGCCTA)

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH-stsgRNA2.1

   Plasmid

   #64117

   Purpose

   Vector for expression of St1 sgRNA2.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU51

   Plasmid

   #167677

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUB4-2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9–mKate2ps–T1gRNA

   Plasmid

   #62717

   Purpose

   t1 gRNA (ATGAGAATCAAGGCGGTCGA)

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-SSFV-dCas9-GFP

    Plasmid

    #64105

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SSFV

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid

    #68405

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB63

    Plasmid

    #47945

    Purpose

    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_m4_VPR_Puro

    Plasmid

    #167887

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA AAVS1

    Plasmid

    #128119

    Purpose

    Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA AAVS1 + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTX172

    Plasmid

    #89259

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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