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pTol2-10xUAS-Voltron-ST

Plasmid

#119040

Purpose

UAS-driven expresion of soma-localized Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Use

Zebrafish expression

Promoter

E1b

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_Halo_GluA2

Plasmid

#170454

Purpose

Expresses halo tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP E2F6

Plasmid

#236441

Purpose

transient overexpression of E2F6 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F6 (E2F6 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pTol2-10xUAS-Voltron

Plasmid

#119039

Purpose

UAS-driven expresion of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Promoter

E1b

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-cfGFP

Plasmid

#200773

Purpose

Expresses cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

cysteine-free GFP

Use

AAV

Promoter

Synapsin

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHW581

Plasmid

#198818

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-Voltron-ST

Plasmid

#119038

Purpose

Pan neuronal expression of soma-localized Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Use

Zebrafish expression

Promoter

elavl3

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#178821

Purpose

Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRIff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron-ST

Plasmid

#119034

Purpose

Express soma-localized Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53

Plasmid

#221317

Purpose

Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S269D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-mRuby3

Plasmid

#179727

Purpose

Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYP (Syp Mouse)

Use

Lentiviral

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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