We narrowed to 17,748 results for: REV

1. pCfB8709

   Plasmid

   #175224

   Purpose

   Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YOL092W (CEN.PK strain)
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPlacPbuCas13b-gRNA-NT

   Plasmid

   #184838

   Purpose

   Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

   Plasmid

   #106462

   Purpose

   Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(N163,242Q)
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   changed Asparagines at positions 163 and 242 to G…

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

   Plasmid

   #106466

   Purpose

   Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

   Plasmid

   #106467

   Purpose

   Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(H474A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Histidine 474 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

   Plasmid

   #106468

   Purpose

   Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(D415A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Aspartate 415 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LCV2 IRF3 KO

   Plasmid

   #217446

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting IRF3 (IRF3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK5712

   Plasmid

   #219752

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnH3H_v2 - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNK5867

    Plasmid

    #219751

    Purpose

    pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - mcitHispS - tAOX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNK1508

    Plasmid

    #219750

    Purpose

    pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - npgA - tAOX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEL16

    Plasmid

    #107922

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104095

    Purpose

    Expresses TREAT-siRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104094

    Purpose

    Expresses TREAT reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid

    #198245

    Purpose

    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted F-actin binding site on a-catenin (849-90…

    Promoter

    CMV

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. beta catenin binding mutant of alpha catenin sensor

    Plasmid

    #71711

    Purpose

    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin Δβ-cat mutant FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 48- 163

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104096

    Purpose

    Expresses TREAT-ARE reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMEL10

    Plasmid

    #107916

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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