We narrowed to 22,370 results for: ESC;

1. EFS eGFP hPGK BSD

   Plasmid

   #188520

   Purpose

   Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-NHERF1-PDZ2

   Plasmid

   #28296

   Depositor

   Maria-Magdalena Georgescu

   Article

   Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

   Insert

   NHERF1 (NHERF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains aa 148-298 (deletion of the first PDZ do…

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

   Plasmid

   #133342

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM41-EcPPXc

   Plasmid

   #38329

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available Since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pZA-GFP-R5

   Plasmid

   #106256

   Purpose

   Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

   Depositor

   Urartu Seker

   Article

   Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

   Insert

   Green fluorescent protein
   

   Tags

   Added silaffin R5 peptide to C terminus of GFP af…

   Expression

   Bacterial

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos2-Beta-Catenin-20

   Plasmid

   #57350

   Purpose

   Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Beta-Catenin (Ctnnb1 Mouse)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111926

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nano-lantern(Ca2+)_600/pcDNA3

   Plasmid

   #51982

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-2G
   

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGBW-m4046789

   Plasmid

   #145660

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGBW-m4046921

    Plasmid

    #145755

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046901

    Plasmid

    #145725

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

    Plasmid

    #159895

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
    

    Expression

    Worm

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111927

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMB-TGN61r

    Plasmid

    #61175

    Purpose

    Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtSYP61
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046854

    Plasmid

    #145759

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046832

    Plasmid

    #145691

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJB045 (ZapB-mEos2)

    Plasmid

    #72651

    Purpose

    Inducible expression of ZapB-mEos2 in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    ZapB-mEos2
    

    Tags

    mEos2

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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