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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA-trp-HYB

Plasmid

#64331

Purpose

trp1 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of trp1 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pUC19/NLS-mChe

Plasmid

#183162

Purpose

Construct for transient expression of cell nucleus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

NLS-mCherry

Tags

16 aa nuclear localization signal and 16 aa nucl…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDM053

Plasmid

#216815

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM071

Plasmid

#216817

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-TsCC(B)::KANMX

Plasmid

#176397

Purpose

Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-TsCC(B)

Tags

mScarlet fused to TsCC(B)

Expression

Bacterial and Yeast

Promoter

none

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-rtTA-Antares

Plasmid

#197587

Purpose

Expresses TET-On reverse transactivator labeled with Antares

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

rtTA-P2A-Antares

Tags

Antares

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

CaMKII

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-Golgi

Plasmid

#183169

Purpose

Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Golgi

Tags

AtRER1B, A. thaliana endoplasmic reticulum retrie…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTrex-Neo-tdTomato

Plasmid

#47975

Purpose

Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

tdTomato

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEL035

Plasmid

#137903

Purpose

STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-FXR1-isoform b

Plasmid

#225449

Purpose

Express EGFP-fusion protein of FXR1 isoform b

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform b (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-HaloTag

Plasmid

#188907

Purpose

Expresses synaptic HaloTag

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

SYP (Syp Mouse)

Use

Lentiviral

Tags

HaloTag

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hH1

Plasmid

#102841

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_hH1

Use

Pcr template for stagr inserts

Promoter

hH1

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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ECFP-Bak

Plasmid

#31501

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pXTK025 [Origin_RK2-oriT_PartType1]

Plasmid

#229262

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType1

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

RK2-oriT Origin

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tTA

Plasmid

#193338

Purpose

AAV-mediated expression of tTA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tTA2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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