Addgene

We narrowed to 13,612 results for: NTS

Showing: 3481 - 3510 of 13612 results

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pMOD_A0000

Plasmid

#90997

Purpose

Module A, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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V5-BS2-SENP2 (Nuclear Pore)

Plasmid

#220345

Purpose

Mammalian expression vector BS2 in nuclear pore

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

SENP2 (nuclear pore localized protein) and V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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Cox8x2-CeNL_pcDNA3

Plasmid

#89513

Purpose

Cyan color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMC-J6-dCas9-24XGP41-J9

Plasmid

#202006

Purpose

MoClo level 0 dCas9-24XGP41 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo cloning vector

Tags

GS linkers

Expression

Mutation

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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RSV-betaGal

Plasmid

#24058

Depositor

Herbert Samuels, Frederick Stanley

Article

Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

Insert

LacZ (lacZ E. coli)

Use

Tags

RSV promoter

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha1-AtUBQ10:GFP-T35S

Plasmid

#211836

Purpose

Plant transient expression cassette for GFP (control) under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

GFP control

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

Promoter

UBIQUITIN10

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cox8x2-ReNL_pcDNA3

Plasmid

#89533

Purpose

Red color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pG/ER(G)

Plasmid

#108219

Purpose

Yeast expression vector for human estrogen receptor alpha

Depositor

Didier Picard

Article

Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

Insert

Human estrogen receptor a (ESR1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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Integrin-β6 Headpiece I270C

Plasmid

#159638

Purpose

Contains integrin beta 6 residues 1–474 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

Integrin beta 6 headpiece I270C

Use

Tags

6x His

Expression

Mammalian

Mutation

I270C

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Contains D10A and H840A mutations

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pmNFM

Plasmid

#83126

Purpose

Mouse neurofilament protein M cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFM (Nefm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Tags

Expression

Bacterial

Mutation

see manuscript

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Phi3-iRFP

Plasmid

#106296

Purpose

CMV driven iRFP with n terminal membrane binding domain of GAP43

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

iRFPmem

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-PXN

Plasmid

#112957

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

PXN

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-HASAP1-ST

Plasmid

#138965

Purpose

chemigenetic voltage indicator, soma targeted

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP1-ST

Use

Tags

somatic localization tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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hAur-A D274A

Plasmid

#8511

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hAur-A (AURKA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D274A, catalytically dead mutant

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB-SpCas9

Plasmid

#210533

Purpose

Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

SpCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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LI Intron c1

Plasmid

#54433

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Intron element for construction of silencing vectors (intron1 of AtWRKY33)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCO605

Plasmid

#139738

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, AsNPGA

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

NPGA

Use

Luciferase; Moclo level 0 component

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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Cox8x2-YeNL_pcDNA3

Plasmid

#89517

Purpose

Yellow color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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EB3-mTurquoise2

Plasmid

#98825

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

EB3

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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