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We narrowed to 25,908 results for: Nes

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Showing: 5801 - 5850 of 25908 results

  1. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    Non-targeting sgRNA human (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71830-plasmid-map-sequence-id-131025

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  2. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  24. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  27. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid
    #229510
    Purpose
    Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
    Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
    RbcX2 (RbcX2 Mustard Weed)
    BSD2
    RbcX1
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229510-plasmid-map-sequence-id-460710

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Showing: 5801 - 5850 of 25908 results