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  6. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  7. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  8. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  10. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  12. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  16. pLenti6/V5-DEST-p110 CUX1

    Plasmid
    #100816
    Purpose
    Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag
    Depositor
    Alain Nepveu
    Article
    Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1
    Insert
    Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)
    Use
    Lentiviral
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    contains amino acids 747-1505
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    100816-plasmid-map-sequence-id-189653

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  17. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway entry vector
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  18. pAAV-CaMKIIα-EGFP-CRY2-STIM1(318-450)

    Plasmid
    #248300
    Purpose
    An AAV construct designed to express a CRY2-fused truncated STIM1 fragment (a.a.318–450) under the control of the CaMKIIα promoter.
    Depositor
    Sangkyu Lee
    Article
    Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10
    Insert
    CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 Human)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-317,451-685
    Promoter
    CamKlla
    Available Since
    Feb. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    248300-plasmid-map-sequence-id-501303

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  19. pAAV-CaMKIIα-FLAG-STIM1(238-685)

    Plasmid
    #248299
    Purpose
    An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the CaMKIIα promoter.
    Depositor
    Sangkyu Lee
    Article
    Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10
    Insert
    CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) (STIM1 Human)
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-237
    Promoter
    CamKlla
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    248299-plasmid-map-sequence-id-497318

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  20. pAAV-GfaABC1D-EGFP-CRY2-STIM1(318-450)

    Plasmid
    #248302
    Purpose
    An AAV construct designed to express a CRY2-fused truncated STIM1 fragment (a.a.318–450) under the control of the GfaABC1D promoter.
    Depositor
    Sangkyu Lee
    Article
    Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10
    Insert
    CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 Human)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-317,451-685
    Promoter
    GfaABC1D
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    248302-plasmid-map-sequence-id-497322

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Showing: 2321 - 2340 of 2378 results