We narrowed to 25,240 results for: FER

1. EFS-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222501

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC1-4k-BBclone2

   Plasmid

   #137070

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0323
   P13
   DipA
   Lmp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

   Plasmid

   #198751

   Purpose

   Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - LargeBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE3GV-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222502

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS SARS-CoV-2 Alpha Spike

   Plasmid

   #185691

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Alpha Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: Δ69-70, Δ145, N…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pPBbsr-6080NES(Epac-sensor)

   Plasmid

   #162487

   Purpose

   hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Epac-sensor (RAPGEF3 Synthetic, Human)
   

   Use

   Tags

   YPet and Turquoise2GL

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLD-CAG-DYSF-CFL

   Plasmid

   #131457

   Purpose

   Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

   Depositor

   Michele Calos

   Article

   Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

   Insert

   DYSF and Follistatin (DYSF, FST Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   R15C (in DYSF) and Q2579H (in CFL)

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIZ GFP PODXL delta PBM + FBM

   Plasmid

   #202416

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Lacking DTHL, and H477/R479/S481>AAA mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIGF1_P2 Promoter-FLuc

    Plasmid

    #154267

    Purpose

    Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P2 Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-PIG-METTL14-WT

    Plasmid

    #141118

    Purpose

    Retroviral overexpression of WT METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. TOPO mMecp2-NLucTom

    Plasmid

    #159282

    Purpose

    To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.

    Depositor

    Joost Gribnau

    Article

    Mira-Bontenbal et al Stem Cell Reports. 2022 Jan 29. pii: S22

    Insert

    Mecp2 (Mecp2 Mouse)
    

    Use

    Tags

    Nluciferase-P2A-TdTomato

    Expression

    Mammalian

    Mutation

    Nluciferase-TdTomato fusion

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-PIG-METTL14-R298P

    Plasmid

    #141119

    Purpose

    Retroviral overexpression of mutant METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMiR-CIP2A-miR-375-mutA-E

    Plasmid

    #53754

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A-E (refer to ci…

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pRK5_mEGFP-NONO-TFE3

    Plasmid

    #237641

    Purpose

    For overexpression of mEGFP-NONO-TFE3

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NONO-TFE3 (NONO, TFE3 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5_mEGFP-YAP-MAMLD1

    Plasmid

    #237675

    Purpose

    For overexpression of mEGFP-YAP-MAMLD1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAMLD1 (YAP1, MAMLD1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5_mEGFP-CRTC1-MAML2-KS

    Plasmid

    #237672

    Purpose

    For overexpression of mEGFP-CRTC1-MAML2-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    CRTC1-MAML2-KS (CRTC1, MAML2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5_mEGFP-YAP-MAML2

    Plasmid

    #237674

    Purpose

    For overexpression of mEGFP-YAP-MAML2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAML2 (YAP1, MAML2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5_mEGFP-NUP98-HOXA9-KS

    Plasmid

    #237673

    Purpose

    For overexpression of mEGFP-NUP98-HOXA9-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NUP98-HOXA9-KS (NUP98, HOXA9 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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