We narrowed to 59,329 results for: cloning

1. MXS_CMV::NeoR-bGHpA

   Plasmid

   #62437

   Purpose

   MXS_chaining vector with CMV::NeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Neomycin with CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGF002

   Plasmid

   #48843

   Purpose

   Provides a BASTA resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   p35S::BastaR::t35S
   

   Use

   Golden gate compatible cloning vector

   Mutation

   internal BsaI recognition site in 35S promoter re…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. MXS_PGK::PuroR-bGHpA

   Plasmid

   #62440

   Purpose

   MXS_chaining vector with PGK::PuroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Puromycin with PGK Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 LC3B2 WT

   Plasmid

   #123075

   Purpose

   Gateway entry clone encoding human MAP1LC3B2 wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
   

   Use

   Gateway entry vector / entry clone

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MXS_MCS1

   Plasmid

   #62395

   Purpose

   MXS_chaining vector with MCS1

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSGV Rh Acceptor

   Plasmid

   #64271

   Purpose

   Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. R777-E115 Hs.KRAS

   Plasmid

   #70399

   Purpose

   Gateway ORF clone of human KRAS [NM_004985.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCSh3-mRFP

   Plasmid

   #170983

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-CMVmin:GFP no-pA

   Plasmid

   #80819

   Purpose

   CMVmin-promoted GFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-GFP no pA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCoofy42

    Plasmid

    #55190

    Purpose

    Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-GFPmem no-pA

    Plasmid

    #80817

    Purpose

    GFPmem without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFPcaax no pA p3E
    

    Expression

    Mammalian

    Promoter

    GFP

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MXS_PGK::NeoR-bGHpA

    Plasmid

    #62438

    Purpose

    MXS_chaining vector with PGK::NeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Neomycin with PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-mKate2 no stop

    Plasmid

    #80806

    Purpose

    red fluorophore without stop codon

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    pME-mKate2 no stop
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNH053

    Plasmid

    #42159

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mCherry-RT-mCherry
    

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGF008

    Plasmid

    #48848

    Purpose

    Provides a BASTA resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pNOS::BastaR::tNOS
    

    Use

    Golden gate compatible cloning vector

    Mutation

    chi sequence in BastaR removed by silent substitu…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCoofy47

    Plasmid

    #55188

    Purpose

    Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 SCAF4

    Plasmid

    #122464

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 (SCAF4 Human)
    

    Use

    Gateway cloning

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOGG006

    Plasmid

    #113981

    Purpose

    Golden Gate Level 2 cloning site, position 1 module for vector construction

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Level 2 golden gate cloning vector
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNH041

    Plasmid

    #42153

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    YFP
    

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-mKate2-myc no-pA

    Plasmid

    #80812

    Purpose

    myc epitope fused to C-terminus of mKate2 without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mKateHA p3E
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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