9,096 results

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pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRK5-myc-RhoA-Q63L

Plasmid

#12964

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Tags

Myc

Expression

Mammalian

Mutation

Q63L Constitutively active

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pSicoR p53

Plasmid

#12090

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-cdk5GFP

Plasmid

#1346

Depositor

Li-Huei Tsai

Insert

CDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Publication

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti-sgRNA puro

Plasmid

#104990

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pUC57-TPNOX

Plasmid

#87853

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

TPNOX

Tags

C-terminal FLAG tag with a linker

Expression

Bacterial

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJL-TRBO-G

Plasmid

#80083

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

Depositor

John Lindbo

Publication

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Insert

GFP

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

ChR2

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +2

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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tdTomato-pBAD

Plasmid

#54856

Purpose

Localization: Protein Expression Vector , Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and tdTomato

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Publication

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Expression

Insect

Promoter

Actin5c

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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CD44P pGL3

Plasmid

#19122

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 promoter (CD44 Human)

Use

Non viral cloning vector

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Publication

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV5 human p27

Plasmid

#14049

Depositor

Joan Massague

Publication

Luo et al Nature. 1995 May 11. 375(6527):159-61.

Insert

p27 (CDKN1B Human)

Expression

Mammalian

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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