Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,598 results for: 112

Showing: 2341 - 2360 of 12598 results

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pHAGE-ALK-R401Q

Plasmid

#116112

Purpose

Lentiviral expression of ALK R401Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R401Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

Plasmid

#184556

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA(dPCNA)

Plasmid

#215112

Purpose

Expresses p21-HA(dPCNA) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

CMV (Tet inducible)

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (CH.1.1)

Plasmid

#241112

Purpose

Expresses SARS-CoV-2 spike (CH.1.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (CH.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147E,W152R,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-3a)

Plasmid

#241123

Purpose

Expresses SARS-CoV-2 spike (XBB-3a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-3a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-4a)

Plasmid

#241124

Purpose

Expresses SARS-CoV-2 spike (XBB-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-2a)

Plasmid

#241122

Purpose

Expresses SARS-CoV-2 spike (XBB-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

Plasmid

#202555

Purpose

Synaptotagmin-1 sgRNA2 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

Plasmid

#202556

Purpose

Synaptotagmin-1 sgRNA3 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

Plasmid

#202554

Purpose

Synaptotagmin-1 sgRNA1 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

Plasmid

#184557

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.2 K+ channel [N372B/1R]

Plasmid

#182112

Purpose

Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat). Derived from hybridoma N372B/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-6a)

Plasmid

#241128

Purpose

Expresses SARS-CoV-2 spike (XBB-6a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-6a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-8a)

Plasmid

#241129

Purpose

Expresses SARS-CoV-2 spike (XBB-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-5c)

Plasmid

#241127

Purpose

Expresses SARS-CoV-2 spike (XBB-5c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-5c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-5a)

Plasmid

#241125

Purpose

Expresses SARS-CoV-2 spike (XBB-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27L,T76I,V83A,G142D,Y1…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-5b)

Plasmid

#241126

Purpose

Expresses SARS-CoV-2 spike (XBB-5b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-5b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,T76I,V83A,G142D,Y1…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (XBB-1a)

Plasmid

#241121

Purpose

Expresses SARS-CoV-2 spike (XBB-1a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-1a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (XBB-10b)

Plasmid

#241120

Purpose

Expresses SARS-CoV-2 spike (XBB-10b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-10b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKT-IDH1(R132H)-IRES-Katushka

Plasmid

#124257

Purpose

Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)

Expression

Mammalian

Mutation

Arginine 132 is mutated to Histidine. This mutati…

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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