We narrowed to 2,368 results for: pcas9

1. sgEML4

   Plasmid

   #85568

   Purpose

   Expresses sgRNA targeting human EML4 between exon 13 and 14

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   echinoderm microtubule associated protein like 4 (EML4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

2. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

   Plasmid

   #87109

   Purpose

   BPNLS-Cas9-BPNLS-2AGFP expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS-2A-EGFP
   

   Use

   CRISPR

   Tags

   3xFLAG (on Cas9)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgKRAS(B)

   Plasmid

   #85567

   Purpose

   Expresses sgRNA targeting human KRAS around codon G12

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   KRAS proto-oncogene, GTPase (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

5. sgKRAS(A)

   Plasmid

   #85566

   Purpose

   Expresses sgRNA targeting human KRAS around codon G12

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   KRAS proto-oncogene, GTPase (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00027

   Plasmid

   #199279

   Purpose

   SpCas9 construct to knockout murine Cd274

   Depositor

   Raj Chari

   Article

   Ruf et al Cell. 2023 Aug 17;186(17):3686-3705.e32.

   Insert

   Cd274 guide RNA (Cd274 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

   Plasmid

   #163643

   Purpose

   Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON17 hgRNA
   Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
   

   Use

   Lentiviral

   Tags

   Luc2-P2A-tdTomato-T2A

   Expression

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   EFS and pU6

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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