We narrowed to 6,573 results for: tac

1. pGBT9-hSyntenin2b

   Plasmid

   #89422

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Yeast

   Mutation

   * see below

   Promoter

   ADH1

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVD447

   Plasmid

   #78569

   Purpose

   E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

   Depositor

   James Kaper

   Article

   Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

   Insert

   lee operon
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

3. mCherry-miniSOG-Display-5

   Plasmid

   #54802

   Purpose

   Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry-miniSOG-Display

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. MERS-CoV spike first3KO mutant

   Plasmid

   #231922

   Purpose

   For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike first3KO (S MERS-CoV)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N1176Q, N1213Q, N1225Q

   Promoter

   CMV

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kif1A-EGFP-SNAP

   Plasmid

   #229851

   Purpose

   Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif1A-EGFP-SNAP (Kif1a Rat)
   

   Use

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MERS-CoV spike last4KO mutant

   Plasmid

   #231923

   Purpose

   For expression of MERS-CoV spike protein with glycan deletion at N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike last4KO (S MERS-CoV)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N1241Q, N1256Q, N1277Q, N1288Q

   Promoter

   CMV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SARS-CoV-2 spike G1 mutant

   Plasmid

   #231913

   Purpose

   For expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike G1 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N1098Q

   Promoter

   CMV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH4040

   Plasmid

   #229803

   Purpose

   Pgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures expression of RFP

   Depositor

   Mei Zhen

   Article

   Lin et al Sci Adv. 2023 Mar;9(9):eade1249. doi: 10

   Insert

   Pgpc-1 wCherry unc-54 3' UTR
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pgpc-1

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM201

   Plasmid

   #227660

   Purpose

   pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   3x-FLAG-HRV3C-

   Expression

   Yeast

   Mutation

   (amino acids 121-131) VTENKIF mutated to AAAAAAA

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPY-ymsfGFP

    Plasmid

    #209538

    Purpose

    Expresses CYP tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CPY*-ymsfGFP

    Plasmid

    #209539

    Purpose

    Expresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY*
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Stargazin-EBFP2-FRB

    Plasmid

    #222417

    Purpose

    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Use

    Tags

    EBFP2-HA-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA_SRR124.5_tagBFP

    Plasmid

    #163753

    Purpose

    gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR124.5
    

    Use

    CRISPR

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-coSnaA-TEV-His_His-TEV-SnaC

    Plasmid

    #225059

    Purpose

    recombinant expression of SnaA in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    SnaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_CMV_Prss-Halo-KDEL

    Plasmid

    #216256

    Purpose

    CMV promoter-regulated ER targeted HaloTag expression vector

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER retained HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid

    #221844

    Purpose

    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GACG and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-GFP-LAP2beta

    Plasmid

    #222416

    Purpose

    To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    LAP2beta (Tmpo Rat)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-GFP-BAF

    Plasmid

    #222414

    Purpose

    To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    BAF (BANF1 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210743

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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