2,202 results

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pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -1

Plasmid

#180008

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-cytoBirA-2A-mCherry_Ras

Plasmid

#80062

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Sox10 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

Plasmid

#140893

Purpose

Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

Depositor

Yasushi Okamura

Article

Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

Insert

CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)

Tags

mCherry

Expression

Mammalian

Mutation

changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-SV40

Plasmid

#117809

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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SI10-Brevican

Plasmid

#46300

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Brevican mouse monoclonal antibody (bcan Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
TAL3194

Plasmid

#41368

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Left (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only

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TAL3195

Plasmid

#41369

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Right (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

Plasmid

#119061

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR-zrif1_exon8

Plasmid

#194434

Purpose

Right (RR) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_RIGHT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD-zrif1_exon8

Plasmid

#194435

Purpose

Left (DD) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_LEFT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Tags

GFP

Expression

Bacterial

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ gfp-zASC

Plasmid

#98703

Purpose

GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

Depositor

Anna Huttenlocher

Article

Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

Insert

Apoptosis associated speck like protein containing CARD (pycard Zebrafish)

Use

Zebrafish/xenopus expression

Tags

GFP

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTol1:uas-myc-act-map2k2b-2a-tdTomato

Plasmid

#104721

Purpose

UAS-driven expression of activated MEK

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated map2k2b (map2k2b Zebrafish)

Use

Zebrafish transposon

Tags

2A-tdTomato and myc tag

Mutation

serines 221 and 225 to aspartic acid

Promoter

10xUAS

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-TagBFP

Plasmid

#117765

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3216

Plasmid

#42679

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-left (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pKHR7

Plasmid

#74624

Purpose

a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

cmlc2::mCherry

Use

Zebrafish expression

Promoter

cmlc2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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