2,163 results

1. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Tags

   Expression

   Mutation

   Promoter

   myo6b

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. M58 pPET28b-TEV-full-length zebrafish beta catenin

   Plasmid

   #17199

   Depositor

   Randall Moon

   Insert

   beta catenin (ctnnb1 Zebrafish)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pSYC-97

   Plasmid

   #31565

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   pCS4+-NLS-EGFP-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hox D4a

   Plasmid

   #36469

   Depositor

   Robert Ho

   Article

   Prince et al Development. 1998 Feb . 125(3):407-20.

   Insert

   Hox D4a (hoxd4a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Bacterial

   Mutation

   Q100H, A101T, G125C and R189W

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPRISM-Cre-cmlc2-eGFP

   Plasmid

   #117794

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7tyrgRNA

   Plasmid

   #46761

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   tyr gRNA (tyr Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1:uas-myc-act-map2k2b-2a-tdTomato

   Plasmid

   #104721

   Purpose

   UAS-driven expression of activated MEK

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   activated map2k2b (map2k2b Zebrafish)
   

   Use

   Zebrafish transposon

   Tags

   2A-tdTomato and myc tag

   Expression

   Mutation

   serines 221 and 225 to aspartic acid

   Promoter

   10xUAS

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

   Plasmid

   #172535

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E Lck

   Plasmid

   #135201

   Purpose

   T-cell marker

   Depositor

   David Tobin

   Insert

   Lck promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MiniCoopR mitfa:KIT L576P

    Plasmid

    #118848

    Purpose

    Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT L576P (KIT Human, Zebrafish)
    

    Use

    Zebrafish expression, tol4

    Tags

    Expression

    Mutation

    KIT L576P

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK074 p5E exorh:EGFP

    Plasmid

    #195951

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTol2_claudink:Cas9green; erbb2_gRNA171

    Plasmid

    #199339

    Purpose

    pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. zCREST3:Cas9green; EGF_gRNA

    Plasmid

    #199333

    Purpose

    pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 EGF sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2_claudink:Cas9green; control_gRNA

    Plasmid

    #199336

    Purpose

    pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZE32 Prickle-1-GFP-pCS2+

    Plasmid

    #17073

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-1 (prickle1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pSYC-437

    Plasmid

    #163829

    Purpose

    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

    Depositor

    Seok-Yong Choi

    Article

    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

    Inserts

    Foxj1a Promoter
    GCaMP6s
    

    Use

    Multisite gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-sod1

    Plasmid

    #107594

    Purpose

    Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    sod1 (sod1 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-UAS-Dendra2

    Plasmid

    #97145

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge