2,162 results

1. pCS2+Paxillin-mKate

   Plasmid

   #105974

   Purpose

   in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Paxillin
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2CG2-acta1:QFGal4-SV40pA

   Plasmid

   #155121

   Purpose

   Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   acta1:QFGal4
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   acta1

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-cmlc2-Trpa1b-2A-EGFP

   Plasmid

   #106405

   Purpose

   For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-EGFP

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKE13-ISceI-fabp10a:CreERT2

   Plasmid

   #198242

   Purpose

   For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

   Depositor

   Didier Stainier

   Article

   Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

   Inserts

   Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
   Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST_6xUAS:miRFP670-cetn4

   Plasmid

   #192357

   Purpose

   zfCETN4 tagged with miRFP670, expressed under the UAS promoter

   Depositor

   Darren Gilmour

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   CETN4 (cetn4 Zebrafish)
   

   Use

   Tags

   miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-U6a-U6c-rac2 guide

   Plasmid

   #107600

   Purpose

   An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   rac2 guide RNA sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7tyrgRNA

   Plasmid

   #46761

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   tyr gRNA (tyr Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-Tol2-PA2-CMV-AB-mCh

   Plasmid

   #160435

   Purpose

   Expresses human Amyloid Beta-mCherry in Zebrafish

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Human Amyloid Beta peptide (1-42) (APP Zebrafish, Human)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSYC-97

    Plasmid

    #31565

    Depositor

    Seok-Yong Choi

    Article

    Kim et al PLoS One. 2011 . 6(4):e18556.

    Insert

    pCS4+-NLS-EGFP-P2A-mCherry-CAAX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. shha-pCRII

    Plasmid

    #68962

    Purpose

    for antisense probe, NotI w/ Sp6

    Depositor

    David Traver

    Article

    Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

    Insert

    shha (shha Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. M58 pPET28b-TEV-full-length zebrafish beta catenin

    Plasmid

    #17199

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1 Zebrafish)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

13. MiniCoopR mitfa:KIT L576P

    Plasmid

    #118848

    Purpose

    Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT L576P (KIT Zebrafish, Human)
    

    Use

    Zebrafish expression, tol4

    Tags

    Expression

    Mutation

    KIT L576P

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E Lck

    Plasmid

    #135201

    Purpose

    T-cell marker

    Depositor

    David Tobin

    Insert

    Lck promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPRISM-gal4-VP16-cmlc2-mRFP

    Plasmid

    #117781

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    gal4/VP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2-UAS-Dendra2

    Plasmid

    #97145

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZE32 Prickle-1-GFP-pCS2+

    Plasmid

    #17073

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-1 (prickle1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

    Plasmid

    #172535

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge