We narrowed to 564 results for: POLL

1. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

   Plasmid

   #222107

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-mCherry

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

   Plasmid

   #222108

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-GFP

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

   Plasmid

   #197969

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L1332V, delta(1616-1666)

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

   Plasmid

   #197970

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   C328S, C331S, L1332V, delta(1616-1666)

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-Basic-ZC3H12D_PromoterWT

   Plasmid

   #153065

   Purpose

   ZC3H12D promoter region cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region (ZC3H12D Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUW mCherry-GFP-LC3

   Plasmid

   #110060

   Purpose

   To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

   Depositor

   Anne Brunet

   Article

   Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

   Insert

   mCherry-GFP-LC3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-H1-shScramble-hsynapsin-EGFP

   Plasmid

   #220795

   Purpose

   control shRNA plasmid with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

   Plasmid

   #156449

   Purpose

   For transient expression of human PDS5A tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   human PDS5A (PDS5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

   Plasmid

   #220797

   Purpose

   shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-H1-shSRGAP2A-hsynapsin-EGFP

    Plasmid

    #220798

    Purpose

    shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

    Plasmid

    #156447

    Purpose

    For transient expression of mouse SMC3 tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SMC3 (Smc3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pScalps_puro_mIL-2Rβ

    Plasmid

    #59916

    Purpose

    Expresses mouse interleukin-2 receptor Beta chain

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor beta (Il2rb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pX462-hPRKAA1-gRNA_B

    Plasmid

    #74375

    Purpose

    gRNA_B to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX462-hPRKAA1-gRNA_A

    Plasmid

    #74374

    Purpose

    gRNA_A to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

    Plasmid

    #156443

    Purpose

    For transient expression of mouse BORIS (CTCFL) tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse BORIS (Ctcfl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 Flag MFF iso2 WT

    Plasmid

    #74389

    Purpose

    Flag tagged human MFF isoform 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

    Plasmid

    #156442

    Purpose

    For transient expression of mouse PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5B (Pds5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag MFF iso5 WT

    Plasmid

    #74378

    Purpose

    Flag tagged human MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3 Flag MFF iso1 WT

    Plasmid

    #74388

    Purpose

    Flag tagged human MFF isoform 1

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3 Flag MFF iso4 WT

    Plasmid

    #74390

    Purpose

    Flag tagged human MFF isoform 4

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP

    Plasmid

    #220796

    Purpose

    shRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YCe2741 HC_Kan_EF1ap_p18

    Plasmid

    #100698

    Purpose

    part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    EF1a promoter (EEF1A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScalps_puro_mIL-2Rα ST

    Plasmid

    #59919

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed sequence of the intracellular (C-terminal…

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

    Plasmid

    #156438

    Purpose

    For transient expression of mouse CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse CTCF (Ctcf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti.CMV/TO_PRKAA2

    Plasmid

    #74447

    Purpose

    Lentiviral expression vector encoding untagged WT human AMPK alpha 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    AMPK alpha 2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

    Plasmid

    #156448

    Purpose

    For transient expression of human CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human CTCF (CTCF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

    Plasmid

    #149714

    Purpose

    Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

    Plasmid

    #122954

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

33. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid

    #139457

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid

    #139458

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQCXIB Flag MFF WT

    Plasmid

    #74384

    Purpose

    Retroviral expression vector containing Flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3 Flag MFF iso1 S172A

    Plasmid

    #74391

    Purpose

    Flag tagged human S172A MFF isoform 1 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26) and Ser 172 Ala.

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pScalps_puro_mIL-2Rα RK

    Plasmid

    #59920

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed sequence of the intracellular (C-terminal…

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pCDNA3 NDUFS6(5’UTR)-FF

    Plasmid

    #85488

    Purpose

    Firefly luciferase under the control of NDUFS6 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of NDUFS6 (NDUFS6 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

40. pX330-EN1680_Sororin-Cterm

    Plasmid

    #156451

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, SORORIN (Cdca5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge