Skip to main content
Addgene
Showing: 441 - 480 of 4054 results
  1. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid
    #91781
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Asp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid
    #91740
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Arg (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pFUSEss-CHIg-mM-Asn209Ile_M18

    Plasmid
    #91746
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ile (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pFUSEss-CHIg-mM-Asn209Val_M18

    Plasmid
    #91756
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Val (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid
    #91755
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Tyr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pFUSEss-CHIg-mM-Asn209Thr_M18

    Plasmid
    #91753
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Thr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pFUSEss-CHIg-mM-Lys208Ala_M18

    Plasmid
    #91732
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lys208 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pFUSEss-CHIg-mM-Asn209Trp_M18

    Plasmid
    #91754
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Trp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. pFUSEss-CHIg-mM-Asn209Ser_M18

    Plasmid
    #91752
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ser (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pFUSEss-CHIg-mM-Asn209Pro_M18

    Plasmid
    #91751
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Pro (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Pro (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. pFUSEss-CHIg-mM-Asn209Phe_M18

    Plasmid
    #91750
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Phe (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Phe (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  17. pFUSEss-CHIg-mM-Asn209Met_M18

    Plasmid
    #91749
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Met (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Met (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  18. pFUSEss-CHIg-mM-Asn209Lys_M18

    Plasmid
    #91748
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Lys (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Lys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  19. pFUSEss-CHIg-mM-Asn209Leu_M18

    Plasmid
    #91747
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Leu (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Leu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  20. pFUSEss-CHIg-mM-Asn209His_M18

    Plasmid
    #91745
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209His (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to His (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  21. pFUSEss-CHIg-mM-Asn209Gly_M18

    Plasmid
    #91744
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gly (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gly (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  22. pFUSEss-CHIg-mM-Asn209Glu_M18

    Plasmid
    #91743
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Glu (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Glu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  23. pFUSEss-CHIg-mM-Asn209Gln_M18

    Plasmid
    #91742
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gln (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gln (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  24. pFUSEss-CHIg-mM-Asn209Cys_M18

    Plasmid
    #91741
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Cys (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Cys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  25. pFUSEss-CHIg-mM-Asp212Ser_M18

    Plasmid
    #91739
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asp212 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  26. pFUSEss-CHIg-mM-His214Ala_M18

    Plasmid
    #91737
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His214Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    His214 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  27. pFUSEss-CHIg-mM-Leu213Ala_M18

    Plasmid
    #91736
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Leu213Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Leu213 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  28. pFUSEss-CHIg-mM-Asp212Ala_M18

    Plasmid
    #91735
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asp212 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  29. pFUSEss-CHIg-mM-Tyr205Ala_M18

    Plasmid
    #91731
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Tyr205Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Tyr205 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  30. pFUSEss-CHIg-mM-His204Ala_M18

    Plasmid
    #91730
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His204Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    His204 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  31. pCDB179

    Plasmid
    #91960
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Type
    Empty backbone
    Use
    Tags
    10-His and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  32. pCDB24

    Plasmid
    #91959
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Type
    Empty backbone
    Use
    Tags
    10-His and Smt3
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. pCDH-CMV-human MYCN-EF1-mRFP

    Plasmid
    #193708
    Purpose
    Lentiviral expression of human MYCN
    Depositor
    Insert
    MYCN (MYCN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 441 - 480 of 4054 results