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pLenti-CRISPRv2-sgIl1r1_#2-hygro

Plasmid

#231990

Purpose

Knockout mouse Il1r1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

Plasmid

#154834

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

ETN
ETN

Use

Cre/Lox

Expression

Mammalian

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

Plasmid

#154829

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

coEPO
coEPO

Use

Cre/Lox

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro shSlp4-a #2

Plasmid

#40070

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-2

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4 CoV-2 nsp1 3xFLAG

Plasmid

#176057

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHMTc-FBF-2 (121-632)

Plasmid

#218664

Purpose

Expression of MBP:6xHIS:FBF-2(121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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XLone-UCE intron (DF3-2)

Plasmid

#242186

Purpose

Express doxycycline inducible CRNDE UCE intron with deletion of the eIF6 binding site F3-2 in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE intron (DF3-2) (CRNDE Human)

Expression

Mammalian

Mutation

F3-2 site deleted (70 bp)

Promoter

TRE3GS

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75247

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro shPRDM14-2

Plasmid

#193695

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-ML1N*2

Plasmid

#67665

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1N*2

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shSLC2A1-2

Plasmid

#193703

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 2 DEPTOR

Plasmid

#21336

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 2 (DEPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pLKO mouse shRNA 2 DEPTOR

Plasmid

#21338

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 2 (Deptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro shYAP1-2

Plasmid

#193693

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNkx2-1#2/Cre

Plasmid

#193222

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNkx2-1#2 (Nkx2-1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-2

Plasmid

#193700

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway entry vector

Expression

Mammalian

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSox10-2

Plasmid

#37007

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSox10-2 (Sox10 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
p-eGFP-beta 2 CP

Plasmid

#13298

Depositor

John Cooper

Article

Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

Insert

capping protein beta 2 subunit (Capzb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44s (fl)

Plasmid

#137812

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length, I186T and I253V on CD44

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6 (fl)

Plasmid

#137816

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

R296K in the V6 region [Please see depositor comm…

Promoter

PGK

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v9 (fl)

Plasmid

#137818

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v9 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length and mutation H48L

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v3 (fl)

Plasmid

#137814

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length and N280D in the V3 region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44s (-cyt)

Plasmid

#137815

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v9 (-cyt)

Plasmid

#137819

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v9 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #2

Plasmid

#179455

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v6 (-cyt)

Plasmid

#137817

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region, R296K (please see dep…

Promoter

PGK

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v3 (-cyt)

Plasmid

#137813

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region, N280D in the V3 region

Promoter

PGK

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

Plasmid

#173986

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #2

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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We narrowed to 12,481 results for: cel.2;