Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 75,765 results for: Rest

Showing: 551 - 600 of 75765 results

  2. pQL269

    Plasmid
    #59802
    Purpose
    The univector plasmid-fusion system (UPS) uses Cre– lox site-specific recombination to fuse a plasmid containing the gene of interest and host vectors containing regulatory information.
    Depositor
    Stephen Elledge
    Article
    Liu et al Curr Biol. 1998 Dec 3;8(24):1300-9.
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lambdaPR
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pSC-LPETG

    Plasmid
    #207629
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-LPETG
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207629-plasmid-map-sequence-id-409853

    Enlarge

  9. OfCasp3b

    Plasmid
    #166054
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3b
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166054-plasmid-map-sequence-id-328273

    Enlarge

  10. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

    Enlarge

  11. PaCasp3

    Plasmid
    #166052
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-3
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166052-plasmid-map-sequence-id-328270

    Enlarge

  12. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

    Enlarge

  17. pSB1A3 EcoRI Methylase-AmilCP

    Plasmid
    #85166
    Purpose
    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI Methylase-AmilCP
    Use
    Tags
    AmilCP
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pSC-cUnaG-LPETG

    Plasmid
    #207635
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207635-plasmid-map-sequence-id-409865

    Enlarge

  19. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

    Enlarge

  22. EGFP-ST-P2A-pSC-CAAX

    Plasmid
    #207637
    Purpose
    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207637-plasmid-map-sequence-id-409879

    Enlarge

  26. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

    Enlarge

  28. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

    Enlarge

  43. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

    Enlarge

  45. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

    Enlarge

Showing: 551 - 600 of 75765 results