We narrowed to 2,196 results for: LOB;

1. pGEX6P1‐Afu‐rnhBΔPIP

   Plasmid

   #108696

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-terminal truncation (7aa) removing PIP box

   Promoter

   tac

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b+PCNA3 R112C/T180C

   Plasmid

   #66182

   Purpose

   Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C, T180C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b+PCNA2 E105C/L171C

   Plasmid

   #66178

   Purpose

   Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C, L171C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pt NaV1.4

   Plasmid

   #200233

   Purpose

   Expression in mammalian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

   Insert

   Phyllobates terriblis NaV1.4 – muscle channel
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMVd1-NLS-GAL4-iLight-VP16

   Plasmid

   #219549

   Purpose

   NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight-VP16
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVd1

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAS-GFP::NMN-Deamidase{dead}

   Plasmid

   #187869

   Purpose

   Gal4/UAS expression of GFP::NMN-Deamidase(dead)

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   NMN-Deamidase{dead}
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-4x(tRNA::axed{sgRNA})

   Plasmid

   #187883

   Purpose

   Gal4/UAS sgRNA expression targeting axed

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   4 sgRNAs targeting axed
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. UAS-4x(tRNA::hiw{sgRNA})

   Plasmid

   #187884

   Purpose

   Gal4/UAS sgRNA expression targeting hiw

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   4 sgRNAs targeting hiw
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS-4x(tRNA::dsarm{sgRNA})

    Plasmid

    #187885

    Purpose

    Gal4/UAS sgRNA expression targeting dsarm

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    4 sgRNAs targeting dSarm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

    Plasmid

    #186418

    Purpose

    Expression of scrambled shRNA used as a control for knock down experiments

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    ScramblemiR
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

    Plasmid

    #186419

    Purpose

    Expression of shRNA for the knock down of mouse Egr3

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    miREgr3-5D
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDUAL_2_lacZ

    Plasmid

    #155173

    Purpose

    see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDUAL_araBAD

    Plasmid

    #155172

    Purpose

    BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDUAL_2_tacI

    Plasmid

    #155174

    Purpose

    see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDUAL_lacZ

    Plasmid

    #155169

    Purpose

    BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBICI_2_medium

    Plasmid

    #155167

    Purpose

    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBICI_strong

    Plasmid

    #155165

    Purpose

    BaSP and CuCBP co-expression: strong RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBICI_medium

    Plasmid

    #155164

    Purpose

    BaSP and CuCBP co-expression: medium RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBICI_weak

    Plasmid

    #155163

    Purpose

    BaSP and CuCBP co-expression: weak RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge