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h-alpha-ENaC-2

Plasmid

#83431

Purpose

full length human alpha ENaC-2 with flag tag

Depositor

Christie Thomas

Article

Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

Insert

human alpha ENaC isoform 2 (SCNN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDsRed-HP1alpha

Plasmid

#179702

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

Insert

heterochromatin protein alpha (CBX5 Human)

Use

Tags

dsRed

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha2_SF (GB0107)

Plasmid

#68233

Purpose

Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FusionRed-BaxAlpha-C-15

Plasmid

#56101

Purpose

Localization: , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

BaxAlpha

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRSETA-alphaFlincG

Plasmid

#19735

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

alpha-FlincG

Use

Protein expression

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pR6Kg-Alpha1spm

Plasmid

#165866

Purpose

Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMADM-alpha

Plasmid

#36890

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, deleted…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAO1_G-alpha

Plasmid

#110013

Purpose

Protein expression and purification of GNAO1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAO1_G-alpha (GNAO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNA13_G-alpha

Plasmid

#109913

Purpose

Protein expression and purification of GNA13_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA13_G-alpha (GNA13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAT1_G-alpha

Plasmid

#109946

Purpose

Protein expression and purification of GNAT1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAT1_G-alpha (GNAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAZ_G-alpha

Plasmid

#109949

Purpose

Protein expression and purification of GNAZ_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAZ_G-alpha (GNAZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pNTPTP alpha

Plasmid

#17696

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Galpha-o

Plasmid

#15930

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-o (Gnao1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Tags

Expression

Mutation

changed sequence of the intracellular (C-terminal…

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pCR-HA-IKKalphad17

Plasmid

#19738

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Splice variant lacking 17 amino acids at the C-te…

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pCR2.1-p55alpha

Plasmid

#1409

Depositor

Lewis Cantley

Insert

PI3K regulatory subunit p55alpha (Pik3r1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
Alt AlphaPS3

Plasmid

#14070

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Alt Alpha PS3 Integrin (scb Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBS Calponin3

Plasmid

#19194

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calponin 3 (CNN3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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PT7-alphaHL

Plasmid

#227650

Purpose

pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.

Depositor

Richard Murray

Insert

alpha-hemolysin

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge