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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Use

Tags

V5

Expression

Bacterial and Insect

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ADARcd-YTHD422N

Plasmid

#194402

Purpose

ADAR1 catalytic domain E488Q fused to YTHD422N

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

ADARcd fused to YTHD422N (ADAR Human)

Use

Tags

ADARcd-YTHD422N-HA

Expression

Mammalian

Mutation

D422N mutation in the YTH domain to enhance m6A b…

Promoter

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon

Plasmid

#126941

Purpose

Expression of SomArchon under Syn promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC403 SMARCB1 var 2

Plasmid

#111185

Purpose

Inducible vector (V5 tagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

TRE

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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B270 + SMARCAL1 sgSTOP

Plasmid

#100717

Purpose

B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 WC visual barcode

Plasmid

#158666

Purpose

tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-PARC

Plasmid

#20937

Depositor

Yue Xiong

Insert

p53-associated parkin-like cytoplasmic protein (CUL9 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

S1049N, C1076R, R2389L

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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B52 + SMARCAL1 sgSTOP

Plasmid

#100715

Purpose

B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SMARCAL1 (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight A173

Plasmid

#53615

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-A173

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
acGFP WC visual barcode

Plasmid

#158672

Purpose

tagging clonal populations with whole cell acGFP fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK14-P2A-Hygro_Barcode

Plasmid

#170239

Purpose

Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK14 (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-C-mCherryTag

Plasmid

#218183

Purpose

To tag Hevin with mCherry at its C-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ADARcd-YTHmut

Plasmid

#194431

Purpose

ADAR1 catalytic domain E488Q fused to YTHmut

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

ADARcd fused to YTHmut domain (ADAR Human)

Use

Tags

ADARcd-YTHmut-HA

Expression

Mammalian

Mutation

m6A binding region of YTH domain is deleted

Promoter

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 WC visual barcode

Plasmid

#158669

Purpose

tagging clonal populations with whole cell mTurquoise2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSMARCA4 guide 1

Plasmid

#193604

Purpose

SMARCA4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 1 (SMARCA4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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