We narrowed to 4,639 results for: crispr c plasmids

1. pCX138

   Plasmid

   #229213

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 25-48)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCX459

   Plasmid

   #229214

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCX460

   Plasmid

   #229215

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 25-48)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZNF785-donor

   Plasmid

   #112317

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

   Depositor

   Kevin White

   Insert

   ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBHM2641

   Plasmid

   #229525

   Purpose

   Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   CRISPR

   Tags

   t2a-mNeonGreen-AID*

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   TEF1

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD362

   Plasmid

   #137023

   Purpose

   SapTrap HaloTag-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized Halo Tag
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized Halo Tag

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF416-donor

   Plasmid

   #112372

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF416 with GFP

   Depositor

   Kevin White

   Insert

   ZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:19:57572882:A>G,hg38:19:57572932:C>G,h…

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZNF766-donor

   Plasmid

   #112352

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF766 with GFP

   Depositor

   Kevin White

   Insert

   ZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:19:52290357:G>T, hg38:19:52290373:A>G,…

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDLX1-donor

    Plasmid

    #112343

    Purpose

    CRISPR donor plasmid to tag human transcription factor DLX1 with GFP

    Depositor

    Kevin White

    Insert

    DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. peft-3::cas-9::tbb-2 3'UTR

    Plasmid

    #48960

    Purpose

    this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK02

    Plasmid

    #105133

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKM126

    Plasmid

    #105134

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN10

    Plasmid

    #114386

    Purpose

    Empty gRNA

    Depositor

    Vincent Dion

    Article

    Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge