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We narrowed to 4,639 results for: crispr c plasmids

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Showing: 221 - 240 of 4639 results

  1. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  2. pZNF416-donor

    Plasmid
    #112372
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF416 with GFP
    Depositor
    Kevin White
    Insert
    ZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
    Use
    CRISPR
    Mutation
    hg38:19:57572882:A>G,hg38:19:57572932:C>G,h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112372-plasmid-map-sequence-id-229731

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  3. pZNF766-donor

    Plasmid
    #112352
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF766 with GFP
    Depositor
    Kevin White
    Insert
    ZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
    Use
    CRISPR
    Mutation
    hg38:19:52290357:G>T, hg38:19:52290373:A>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112352-plasmid-map-sequence-id-229667

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  4. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  5. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  6. pX330-U6-MYC-Guide-hSpCas9

    Plasmid
    #228574
    Purpose
    Expression of human MYC gRNA; CRISPR mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    MYC gRNA (MYC Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228574-plasmid-map-sequence-id-457356

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  8. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  9. pDLX1-donor

    Plasmid
    #112343
    Purpose
    CRISPR donor plasmid to tag human transcription factor DLX1 with GFP
    Depositor
    Kevin White
    Insert
    DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
    Use
    CRISPR
    Mutation
    hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112343-plasmid-map-sequence-id-229657

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  11. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  12. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  13. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

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  16. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  17. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Kevin White
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112377-plasmid-map-sequence-id-229742

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  18. pMLX-donor

    Plasmid
    #112370
    Purpose
    CRISPR donor plasmid to tag human transcription factor MLX with GFP
    Depositor
    Kevin White
    Insert
    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    Use
    CRISPR
    Mutation
    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112370-plasmid-map-sequence-id-229723

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  19. pTHRB-donor

    Plasmid
    #112333
    Purpose
    CRISPR donor plasmid to tag human transcription factor THRB with GFP
    Depositor
    Kevin White
    Insert
    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    Use
    CRISPR
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112333-plasmid-map-sequence-id-228563

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  20. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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Showing: 221 - 240 of 4639 results