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Plasmid#160377PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA9-Halo
Plasmid#175332PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA9 (HSPA9 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP2 (IGF2BP2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pH6HTC_HNRNPA1-Halo
Plasmid#175307PurposeProduction of HaloTag fusion proteinDepositorInsertHNRNPA1 (HNRNPA1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_DIO_mMeCP2-HaloTag
Plasmid#164062PurposeDouble-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoterDepositorInsertMeCP2 Isoform 1 (Mecp2 Mouse)
UseAAVTagsHaloTagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FSCN1-Halo
Plasmid#175343PurposeProduction of HaloTag fusion proteinDepositorInsertFSCN1 (FSCN1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMal-Halo
Plasmid#209349PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3-Halo
Plasmid#175310PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP3 (IGF2BP3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-p58
Plasmid#166896Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTagDepositorInsertp58 (Lman1 Rat)
UseTagsHaloTagExpressionMammalianMutationL405Q, R419S- please see depositor commentsPromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TL023_lenti_CMV:HaloTag-GluA1
Plasmid#192518PurposeExpression of AMPA GluA1 with N terminal HaloTag fusionDepositorInsertHaloTag-GluA1
UseLentiviralTagsHaloTagExpressionMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorInsertMANF (MANF Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-Halo
Plasmid#184417Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-2xrGBD
Plasmid#176109PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
WDR5-HaloTag
Plasmid#200881PurposeMammalian expression of human WDR5 with C-terminal HaloTag fusionDepositorInsertWDR5-HaloTag (WDR5 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^Halo
Plasmid#174108PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.DepositorInsertCodon optimized VPS13D
UseTagsHaloExpressionMammalianMutationWith an internal Halo tag in aminoacid position 1…PromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.TfR-Halo
Plasmid#162716PurposeMammalian expression of human TFRCDepositorInsertTfR (TFRC Human) (TFRC Human)
UseTagsHaloExpressionMammalianMutationnonePromoterChicken Beta actinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC59-Halo
Plasmid#175327PurposeProduction of HaloTag fusion proteinDepositorInsertCCDC59 (CCDC59 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HNRNPC-Halo
Plasmid#175306PurposeProduction of HaloTag fusion proteinDepositorInsertHNRNPC (HNRNPC Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only