Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,268 results for: piggyBac

Clear keywords
Showing: 221 - 240 of 2268 results

  4. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid
    #209081
    Purpose
    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression
    Depositor
    Vikram Khurana
    Publication
    Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    A53T, NAC deletion (aa60-95)
    Promoter
    TRE4G
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209081-plasmid-map-sequence-id-416524

    Enlarge

  5. PiggyBac CAG eGFP-PolQ10A-FLAG P2A-BLAST

    Plasmid
    #249812
    Purpose
    Expresses the polymerase theta protein fused with N-terminal eGFP and C-terminal FLAG tag and mutated at serines 1289, 1482, 1486, 1488, 1493, 1555, 1563, 1628, 1635 and threonine 1755 into alanines
    Depositor
    Raphael Ceccaldi
    Publication
    Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:
    Insert
    Polymerase Theta (POLQ Human)
    Tags
    FLAG and eGFP
    Expression
    Mammalian
    Mutation
    serines 1289, 1482, 1486, 1488, 1493, 1555, 1563,…
    Promoter
    CAG
    Available since
    March 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    249812-plasmid-map-sequence-id-502074

    Enlarge

  8. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84239-plasmid-map-sequence-id-160811

    Enlarge

  11. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83961-plasmid-map-sequence-id-214594

    Enlarge

  13. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171379
    Purpose
    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized Cas13X.1
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171379-plasmid-map-sequence-id-337344

    Enlarge

  16. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid
    #190181
    Purpose
    Fluorescent reporter vector to visualize all of cell cycle phases.
    Depositor
    Toshiro Sato
    Publication
    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    190181-plasmid-map-sequence-id-378849

    Enlarge

  17. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84241
    Purpose
    Expresses ABA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84241-plasmid-map-sequence-id-160882

    Enlarge

  18. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid
    #107307
    Purpose
    Encoding an SPH activation system
    Depositor
    Hui Yang
    Publication
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    Expression
    Mammalian
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107307-plasmid-map-sequence-id-457328

    Enlarge

  20. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162279-plasmid-map-sequence-id-321873

    Enlarge

Showing: 221 - 240 of 2268 results