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psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Casper Hoogenraad, Thomas Schwarz

Article

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Insert

trim46 (Trim46 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

Plasmid

#159903

Purpose

Mutagenesis of Oprk1

Depositor

Larry Zweifel

Insert

Oprk1 (Oprk1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDHa_mDDX5_FL_wt

Plasmid

#88869

Purpose

constitutive expression of Ha-DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
HA-Vim

Plasmid

#46315

Depositor

Xiangli Yang

Article

Lian et al J Biol Chem. 2009 Oct 30;284(44):30518-2

Insert

Vimentin (Vim Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pET28b_RanWT

Plasmid

#186270

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-iRFP670-N1

Plasmid

#197227

Purpose

Expresses the fusion protein of H2B-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-iRFP670

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo:Beta2meGFP

Plasmid

#45096

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 (Chrnb2 Mouse)

Expression

Mammalian

Mutation

There are strain differences between C57BJ/6 and …

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-Cep290

Plasmid

#27380

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

please see depositor comments below

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Expression

Bacterial

Mutation

K1750A

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

Plasmid

#159583

Purpose

Luciferase reporter for ribosome pausing

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

XBP1u pause site
EIF2S3 5UTR

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only