We narrowed to 10,315 results for: plasmids 101

1. pAAV-nEF-Con/Fon TVA-mCherry

   Plasmid

   #131779

   Purpose

   INTRSECT 2.0

   Depositor

   Karl Deisseroth

   Article

   Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

   Insert

   TVA-mCherry
   

   Use

   AAV; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

   Plasmid

   #55202

   Purpose

   Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-ChRmine-mScarlet

   Plasmid

   #137158

   Purpose

   Viral expression of ChRmine-p2a-mScarlet

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   ChRmine-p2a-mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119946

   Purpose

   Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 GfaABC1D hM4D mCherry SV40

   Plasmid

   #92286

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   hM4D (CHRM4 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

   Plasmid

   #132686

   Purpose

   AAV plasmid encoding mouse ACY1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Acylase I (Acy1, Acy1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-NpHR3.3-EYFP

   Plasmid

   #137151

   Purpose

   Viral expression of NpHR3.3-EYFP

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   NpHR3.3-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   W179F

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

   Plasmid

   #127868

   Purpose

   pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1 (Nos1 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Amino acids 1-133 only

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NME2 gRNA (BRDN0001487089)

    Plasmid

    #77932

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NME2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NME1-NME2,NME2 (NME2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV8-hSyn-flex-miR30-eGFP-shNts

    Plasmid

    #132717

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shNts (Nts Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. NME2 gRNA (BRDN0001146233)

    Plasmid

    #77933

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NME2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NME1-NME2,NME2 (NME2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TP53RK gRNA (BRDN0001145338)

    Plasmid

    #77699

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TP53RK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TP53RK (TP53RK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TP53RK gRNA (BRDN0001146963)

    Plasmid

    #77700

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TP53RK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TP53RK (TP53RK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TP53RK gRNA (BRDN0001146381)

    Plasmid

    #77701

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TP53RK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TP53RK (TP53RK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STYK1 gRNA (BRDN0001145785)

    Plasmid

    #75695

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STYK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STYK1 (STYK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge