163,682 results

1. psLIB CMV

   Plasmid

   #229953

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6p1-GST-Ubiquitin

   Plasmid

   #223236

   Purpose

   Bacterial expression construct for GST-Tagged Ubiquitin production and purification

   Depositor

   Weixing Zhao

   Insert

   Ubiquitin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-GFP-TRPML1(PHD)

   Plasmid

   #221752

   Purpose

   Stable expression of GFP-TRPML1(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   TRPML1 (MCOLN1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem TRPML1(PHD) (amino acids 1–69)

   Promoter

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMC00029 (aka pRC0610)

   Plasmid

   #200834

   Purpose

   Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3372

   Plasmid

   #144848

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DAXX (DAXX Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GG-EBNA-MIR302-7guides-PGK-Puro

   Plasmid

   #201960

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

   Depositor

   Timo Otonkoski

   Article

   Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

   Insert

   MIR302-7g-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80l ACA

   Plasmid

   #196471

   Purpose

   Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   ACA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEN-CoV2-nanobody-C7

   Plasmid

   #194591

   Purpose

   Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   C7 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWUR702

   Plasmid

   #53079

   Purpose

   Inducible TtAgo expression

   Depositor

   John van der Oost

   Article

   Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

   Insert

   TtAgo
   

   Use

   Tags

   Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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