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pIRES-hrGFP II-TET1_CD

Plasmid

#83570

Purpose

Expresses the catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.hLHX6

Plasmid

#161828

Purpose

Expresses human LHX6(LIM Homeobox 6) in mammalian cells

Depositor

Daniella Ottosson

Article

Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

Insert

human LHX6 (LHX6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AA 17-23 deletion

Promoter

PGK

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBH-UAS-secA5-YFP

Plasmid

#32359

Purpose

Live imaging of apoptotic cells in zebrafish

Depositor

Randall Peterson

Article

van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

Insert

secreted human annexin 5 (ANXA5 Human)

Use

Zebrafish expression

Promoter

UAS

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pES.42.1c

Plasmid

#69576

Purpose

TCR a chain cDNA in pES4 (transgenic construct) for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Expression

Mammalian

Promoter

H-2Kb

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF0608

Plasmid

#142659

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAFA (MAFA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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YSBE 731 : pHYG_4xFLAG

Plasmid

#92090

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

4xFlag

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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