We narrowed to 9,416 results for: Coli

1. SepOTSδ

   Plasmid

   #68285

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 1xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pASKt15C+SrtA5woH

   Plasmid

   #65019

   Purpose

   Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, D160N, D165A, K19…

   Promoter

   tetracycline promoter/operator

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL 1148

   Plasmid

   #53006

   Purpose

   pCon:OxyS, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   OxyS sRNA
   sodB
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon

   Promoter

   pCon and pLlacO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pHL 1057

   Plasmid

   #53001

   Purpose

   pCon:RhyB, pLlacO-1:rpoS::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   rpoS
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   containsn −150 to +30 bp relative to start codon

   Promoter

   pCon and pLlacO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pHL1561

   Plasmid

   #37571

   Depositor

   Han Lim

   Article

   Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

   Insert

   CsrA (csrA E. coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available Since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pHL1559

   Plasmid

   #37570

   Depositor

   Han Lim

   Article

   Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

   Insert

   CsrB (csrB E. coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available Since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pHL1324

   Plasmid

   #37638

   Depositor

   Han Lim

   Article

   Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

   Inserts

   GFP
   TetR
   CsrA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pCon and pConNoHind

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. HL 3076 (=HL 716 + pHL 1069)

   Plasmid

   #30036

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Insert

   pCon:MicC, pLlacO-1:sodB::gfp
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

9. HL 4179 (=HL 865 + pHL 1381)

   Plasmid

   #30098

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Insert

   pLlacO-1:hfq, pLtetO-1:rpoS::gfp
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

10. HL 4202 (=HL 716 + pHL 1389)

    Plasmid

    #30100

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:DsrA, pLlacO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

11. HL 4178 (=HL 862 + pHL 1360)

    Plasmid

    #30097

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLlacO-1:hfq, pLtetO-1:ompC::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pSL5 SMR1713

    Plasmid

    #28028

    Depositor

    Susan Rosenberg

    Article

    Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

    Insert

    MutL (mutL E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pRSET FLII282Pglu-4m

    Plasmid

    #13564

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A T282S-ECFP-N283R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESdelta 799

    Plasmid

    #13042

    Depositor

    Peggy Hsieh

    Article

    Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

    Insert

    MutS
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C-terminal deletion from aa 800 through 853.

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKSI-1

    Plasmid

    #51725

    Purpose

    cloning backbone

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGM1190

    Plasmid

    #69994

    Purpose

    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSFDUET-1_VNp-anti-Lysozyme fAb

    Plasmid

    #214951

    Purpose

    Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-Lysozyme Fab heavy chain fusion
    VNp-anti-Lysozyme Fab light chain fusion
    

    Use

    Tags

    VNp

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSNA

    Plasmid

    #135192

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCKmatBC

    Plasmid

    #138587

    Purpose

    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    matB contains native GUG start codon

    Promoter

    Plac promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Mitochondrial PancACe

    Plasmid

    #215710

    Purpose

    Expresses acetyl-CoA biosensor in the mitochondria

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and mitochondrial localization sequence…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET30a-GlmU-NahK-ET64

    Plasmid

    #234663

    Purpose

    Expression an ELP-tagged fusion enzyme of GlmU and NahK

    Depositor

    Xianwei Liu

    Article

    Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

    Inserts

    N-acetylglucosamine- 1-phosphate uridyltransferase
    N-acetylhexosamine 1-kinase
    

    Use

    Tags

    ELP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge