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pBbdCas9S_arr2

Plasmid

#149639

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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RhoJ

Plasmid

#55696

Purpose

expression of mouse RhoJ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoJ (Rhoj Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMIG small T 110

Plasmid

#9061

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

contains only the N-termial 110 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
Flag-FOXD4

Plasmid

#153119

Purpose

Expression of Flag tagged human FOXD4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4 (FOXD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
IG101: pMVP (L5-L4) TRE promoter

Plasmid

#121729

Purpose

pMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G_arr2

Plasmid

#149571

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G(RBSmut)_arr2

Plasmid

#149578

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-DAT R60A

Plasmid

#90247

Purpose

Expresses YFP-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

mutated R60 to A in human DAT cDNA

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hmga2 m4

Plasmid

#14791

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m4 (Hmga2 Mouse)

Expression

Mammalian

Mutation

Hmga2 m4 mutant (see article and author's ma…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
p3E-rab5b

Plasmid

#109718

Purpose

Multisite gateway vector for 3' tagging with rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab7b

Plasmid

#109724

Purpose

Multisite gateway vector for 3' tagging with rab7b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7b (rab7b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab11al

Plasmid

#109663

Purpose

Multisite gateway vector for 3' tagging with rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A

Plasmid

#24607

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A, C716A

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBbdCas9K(-10TC)_arr2

Plasmid

#149584

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9K_arr2

Plasmid

#149570

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9K(RBSmut)_arr2

Plasmid

#149577

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-ARF5a

Plasmid

#67499

Purpose

Expression of shRNA targeting human ARF5a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5b

Plasmid

#67500

Purpose

Expression of shRNA targeting human ARF5b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 26;184(2):297-308.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Wnt1 Non-STOP

Plasmid

#35887

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab6a

Plasmid

#109720

Purpose

Multisite gateway vector for 3' tagging with rab6a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6a (rab6a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3

Plasmid

#79500

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3 (WNT3 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pES1002

Plasmid

#21789

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Fus3-as2 (FUS3 Budding Yeast)

Expression

Yeast

Mutation

contains 5' 1kb upstream UTR/promoter Q93…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Oncostatin M toehold switch sensor

Plasmid

#111909

Purpose

Toehold switch sensor to detect Oncostatin M mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

Oncostatin M toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-rab6ba

Plasmid

#109721

Purpose

Multisite gateway vector for 3' tagging with rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab6bb

Plasmid

#109722

Purpose

Multisite gateway vector for 3' tagging with rab6bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6bb (rab6bb Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pME-actn2

Plasmid

#109550

Purpose

Multisite gateway vector for 5' tagging with alpha actinin 2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mNG-mCh

Plasmid

#124215

Purpose

Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

mNG-mCh

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-CICf_L40S

Plasmid

#48187

Purpose

Expresses L40S mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Pacsin2

Plasmid

#109587

Purpose

Multisite gateway vector for 3' tagging with Pacsin2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Pacsin2 (pacsin2 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Dnm2b

Plasmid

#109573

Purpose

Multisite gateway vector for 3' tagging with Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab33ba

Plasmid

#109692

Purpose

Multisite gateway vector for 3' tagging with rab33ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab33ba (rab33ba Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab32b

Plasmid

#109622

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab32b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32b (rab32b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab4a-DN

Plasmid

#109735

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab4a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab4a (rab4a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab4a

Plasmid

#109646

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab4a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab4a (rab4a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-CD44a

Plasmid

#109575

Purpose

Multisite gateway vector for 5' tagging with CD44a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44a (cd44a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-CD44a-mKate2

Plasmid

#109523

Purpose

Muscle specific zebrafish expression of mKate2 fused to CD44a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44a (cd44a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-CD44b-mKate2

Plasmid

#109524

Purpose

Muscle specific zebrafish expression of mKate2 fused to CD44b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44b (cd44b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Wnt10A Non-STOP

Plasmid

#35902

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag 3A/myc-M1S46X

Plasmid

#87718

Purpose

Expresses myc-M1S46X spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S46X

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag 3A/myc-M1S44L

Plasmid

#87717

Purpose

Expresses myc-M1S44L spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S44L

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0032-MV_RN

Plasmid

#127015

Purpose

Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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