We narrowed to 4,276 results for: gca

1. AP332-4

   Plasmid

   #66091

   Purpose

   co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs4
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AP332-3

   Plasmid

   #66090

   Purpose

   co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs4
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. AP332-2

   Plasmid

   #66089

   Purpose

   co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs4
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

4. MS2-adRNA (5'GAC)

   Plasmid

   #170127

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(5'GAC)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2-adRNA (3'GAC)

   Plasmid

   #170129

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(3'GAC)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

   Plasmid

   #177335

   Purpose

   bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

   Plasmid

   #207664

   Purpose

   Neuropeptide degradation

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   NEP (MME Human)
   

   Use

   AAV and Cre/Lox

   Tags

   mRUBY3

   Expression

   Mutation

   Addition of signaling peptide (POMC) on the N-ter…

   Promoter

   hSynapsin

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-GEM-GECO1

   Plasmid

   #32442

   Purpose

   Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCascade-IS1

    Plasmid

    #140624

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-sh-hSOX9-1

    Plasmid

    #40644

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-hSOX9-1 (SOX9 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRpuro-sgROSA26

    Plasmid

    #231562

    Purpose

    pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

    Depositor

    Rizwan Haq

    Article

    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

    Insert

    sgROSA26
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF-1a

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.U6.shRNA-Sdc1-1376

    Plasmid

    #195574

    Purpose

    Expresses shRNA to knockdown mouse Syndecan-1

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    shRNA, eGFP (Sdc1 Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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