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We narrowed to 4,241 results for: pes;

Showing: 2401 - 2420 of 4241 results

  1. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid
    #215026
    Purpose
    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Depositor
    Joanna Wysocka
    Article
    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Use
    Luciferase
    Mutation
    4X E-box mutations within Coordinator motifs
    Promoter
    SV40
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    215026-plasmid-map-sequence-id-425844

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  2. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  3. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184875-plasmid-map-sequence-id-366330

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  7. Progerin-S22D-CS-pLPC

    Plasmid
    #73810
    Purpose
    Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.
    Insert
    Progerin (LMNA Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Progerin with substitution of cysteine in CaaX-mo…
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid
    #60394
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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Showing: 2401 - 2420 of 4241 results