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pYPQ145

Plasmid

#69297

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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USP19-Flag/pRK5

Plasmid

#36306

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pLH121

Plasmid

#22696

Depositor

Lynn Hudson

Article

Nadon et al J Neurochem. 1994 Sep . 63(3):822-33.

Insert

Human Proteolipid Protein cDNA (PLP1 Human)

Use

Phagemid

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only
pJH474_pTech_Para-supP

Plasmid

#163718

Purpose

Arabinose-inducible expression of supP

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available Since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only
pGCS-C2m(3xHA)

Plasmid

#85725

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pINS-Blast

Plasmid

#31388

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

bsd

Use

Cre/Lox

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 EGFP-Sumo2 GG

Plasmid

#13384

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 GG (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Mutation

Mature SUMO2GG: diglycine at amino acid positions…

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-SIRT2

Plasmid

#66144

Purpose

Expresses SIRT2 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

Insert

SIRT2 (Sirt2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

E28G

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Article

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Expression

Mammalian

Available Since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PBS/pU6-HIF1 alpha RNAi plasmid 2

Plasmid

#21104

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against hypoxia inducible factor 1alpha

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pXJ40-HA-Merlin I

Plasmid

#19699

Depositor

Filippo Giancotti

Article

Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Insert

Merlin I (Nf2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pMT mCherry-CaaX

Plasmid

#126669

Purpose

Inducible expression of mCherry-CaaX in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

mCherry-CaaX

Expression

Insect

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65

Plasmid

#32569

Depositor

Sergey Kasparov

Article

Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

Inserts

EGFP
Gal4p65

Use

Lentiviral

Promoter

2TPH

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ148

Plasmid

#69300

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAO122 (Apr LEU2 MRC1)

Plasmid

#41923

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-Zeo-ecfp

Plasmid

#65038

Purpose

ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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p3521 pET ubcH6

Plasmid

#8653

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH6 (UBE2E1 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
7851 pUG72-HSC82in

Plasmid

#10894

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Expression

Insect

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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GST-TAZ

Plasmid

#19024

Depositor

Michael Yaffe

Article

Park et al J Biol Chem. 2004 Apr 23. 279(17):17384-

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-miR26a2

Plasmid

#21115

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-26a2 (MIR26A2 Human)

Expression

Mammalian

Available Since

May 18, 2009

Availability

Academic Institutions and Nonprofits only
pTC147

Plasmid

#70013

Purpose

T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

35S:ANT1

Expression

Plant

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shLEF1 a

Plasmid

#27019

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 3a

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pGEARBOCS-v0-Gfap- TagIN-HA

Plasmid

#196491

Purpose

To tag endogenous mouse GFAP with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

HA
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AND-gate-V2.0

Plasmid

#44449

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

AND-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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