We narrowed to 11,368 results for: Vars;

1. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AZ64_pE.DonorCLYBL.TS

   Plasmid

   #199228

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for EGFP reporter
   

   Use

   Gene targeting donor plasmid

   Expression

   Mammalian

   Promoter

   Human PGK1 gene promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-NG-PE2-BSD

   Plasmid

   #176933

   Purpose

   Expresses PE2-NG variant in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Insert

   PE2-NG variant
   

   Use

   Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG HDAC5 WT

   Plasmid

   #32213

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pKP3915

   Plasmid

   #113335

   Purpose

   Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

   Insert

   Artificial Transcription Factor (McIsaac et al, 2013)
   

   Expression

   Yeast

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCerulean-Keratin-17

   Plasmid

   #55379

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Keratin (KRT18 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202561

   Purpose

   hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ECFP-ER-0

   Plasmid

   #55342

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   ER (CALR Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. mCherry-Vimentin-C-18

   Plasmid

   #55157

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

    Plasmid

    #177183

    Purpose

    plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    sgRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY139

    Plasmid

    #223486

    Purpose

    tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    tph-1 (tph-1 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEY82

    Plasmid

    #191077

    Purpose

    flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-7 (flp-7 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-Sig10-Fc-IG

    Plasmid

    #183722

    Purpose

    Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

    Insert

    Siglec-10
    

    Use

    Retroviral

    Tags

    Fc of human IgG1

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ-Myc-FOXA1

    Plasmid

    #232645

    Purpose

    transient expresses FOXA1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Incoherent feedforward (IFF) plasmid

    Plasmid

    #169748

    Purpose

    Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

    Plasmid

    #124621

    Purpose

    Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2(E488Q)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. mTFP1-Vinculin-23

    Plasmid

    #55516

    Purpose

    Localization: Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Vinculin (VCL Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

23. LENC

    Plasmid

    #111163

    Purpose

    miR-E (miR-30 variant)-based RNAi with mCherry reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti-CaMKIIa-Archer1-EGFP

    Plasmid

    #60423

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

    Depositor

    Viviana Gradinaru

    Article

    Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

    Insert

    Archer1-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDAS12342_PEAR-GFP_2in1

    Plasmid

    #177184

    Purpose

    PEAR-GFP plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. xCas9(3.6)-BE4

    Plasmid

    #108386

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE4
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pYDE007

    Plasmid

    #194152

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. PGK+ plasmid with onboard mCherry cassette

    Plasmid

    #169745

    Purpose

    PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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