We narrowed to 13,459 results for: 109

1. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUSEss-CHIg-mG1_Arg322Lys

   Plasmid

   #105849

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG1 heavy chain (Ighg1 Mouse)
   

   Expression

   Mammalian

   Mutation

   Arg322Lys in constant region of IgG1 heavy chain

   Promoter

   hEF1-HTLV

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

   Plasmid

   #175423

   Purpose

   Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 1-117 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   nsp1 C-terminus

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4 3xFLAG Halo delta 118-180 nsp1

   Plasmid

   #175424

   Purpose

   Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 118-180 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminus of nsp1

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

   Plasmid

   #175417

   Purpose

   Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 R124A/K125A nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST47-ARF4-GFP

   Plasmid

   #67392

   Purpose

   Mammalian expression of hArf4 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF4 (ARF4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   V53A, L123P and M134I mutations were unintended P…

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. plko-tet-on-puromycin-shTRX1-259

   Plasmid

   #110920

   Purpose

   To knockdown Thioredoxin-1 following doxycycline addition

   Depositor

   Priyamvada Rai

   Article

   Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

   Insert

   TRX1 (TXN Human)
   

   Use

   Lentiviral

   Promoter

   unknown

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC35F3

   Plasmid

   #132109

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35F3 (SLC35F3 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanR

   Plasmid

   #235109

   Purpose

   Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

   Depositor

   Oscar Ramos

   Article

   Guyot et al bioRxiv 2025.10.29.680610

   Inserts

   Asf1B (ASF1B Human)
   IP3mut3A
   

   Use

   Synthetic Biology; Quantitative bacterial two-hyb…

   Tags

   cI_E34P-rpoA

   Expression

   Bacterial

   Promoter

   LtetO and lpp+lacUV5

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 FLAG FBXO31

    Plasmid

    #236429

    Purpose

    transient overexpression of FBXO31 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

    Insert

    FBXO31 (FBXO31 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid

    #226109

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-92a Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

    Plasmid

    #212109

    Purpose

    Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. fru-T2A-QF2 HDR plasmid

    Plasmid

    #141099

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4 3xFLAG Halo G-Linker nsp1

    Plasmid

    #175426

    Purpose

    Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    G-linker nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    G-linker in place of the RNase destabilization do…

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4 3xFLAG Halo R119A K120A nsp1

    Plasmid

    #175428

    Purpose

    Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 R119A K120A
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants R119A K120A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4 3xFLAG Halo E36A E37A nsp1

    Plasmid

    #175429

    Purpose

    Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E36A E37A
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E36A E37A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Bmpr2 gRNA resistant

    Plasmid

    #163408

    Purpose

    Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    gRNA-targeting sequences are substituted with nuc…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Bmpr2ΔEC gRNA resistant

    Plasmid

    #163413

    Purpose

    Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The extracellular domain (27-150 aa) is deleted. …

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Bmpr2ΔTail gRNA resistant

    Plasmid

    #163412

    Purpose

    Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The C-terminal tail domain (530-1038 aa) is delet…

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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