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pT2K-CAGGS-U6-stuffer (infusion)-IRES-CFP

Plasmid

#114727

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

stuffer (infusion)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pT22cUm5En2E4P3Che(#1188)

Plasmid

#184094

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

5myc-chkEn2-ERT2-5'fP2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22i5uasm5En2E4P3Che(#1208)

Plasmid

#184096

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

5myc-chkEn2-ERT2-5'fP2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …

Promoter

5xUAS

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2069

Plasmid

#141902

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Use

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Tags

Expression

Mutation

Promoter

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA2mt

Plasmid

#58218

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA2mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-Stat5a1*6

Plasmid

#130668

Purpose

Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA6A-EGFR WT

Plasmid

#42665

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR (EGFR Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAIDA1

Plasmid

#79180

Purpose

A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

Depositor

Gen Larsson

Article

Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

Insert

Adhesin Involved in Diffuse Adherence

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG2LMKOSimO

Plasmid

#20866

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Mar 1. ():.

Insert

c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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NLS-SuperNova

Plasmid

#131858

Purpose

Expresses SuperNova fused to nuclear localization signal (NLS)

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

Myc tag and c-myc nuclear localization signal (NL…

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Venus/Cerulean-Synaptopodin-A in pcDNA6

Plasmid

#190798

Purpose

Imaging of synaptopodin in live cells

Depositor

Vivian Tang

Article

Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

Insert

Synaptopodin-A (SYNPO Human, Synthetic)

Use

Tags

Cerulean, His, Venus, and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Orai2

Plasmid

#16369

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai2 (ORAI2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-PTEN-3'UTR-Wt

Plasmid

#21326

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

PTEN 3' UTR Wild Type (PTEN Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLiLucB

Plasmid

#31596

Depositor

Michele Calos

Article

Woodard et al PLoS One. 2010 . 5(6):e11367.

Insert

alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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