We narrowed to 24,482 results for: Spr

1. NM9-SpCas9-NLS3

   Plasmid

   #128177

   Purpose

   Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   SpCas9-NLS3
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pPGK1

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_MCP-LSD1_Hygro

   Plasmid

   #138457

   Purpose

   lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   LSD1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Promoter

   PJ23119 and Pvan

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Promoter

   PJ23119 and Pvan

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCas9-AID

   Plasmid

   #196242

   Purpose

   Plasmid expressing the nCas9-AID fusion

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Insert

   nickase Cas9 fused to AID
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6.3>gRNA.f+e

   Plasmid

   #99139

   Purpose

   Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p201G Cas9

    Plasmid

    #59178

    Purpose

    Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    sGFP
    

    Use

    CRISPR

    Promoter

    2x35S

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-spsgRNA2

    Plasmid

    #64114

    Purpose

    Vector for expression of Sp sgRNA2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp sgRNA2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MiniCoopR U6:gRNA, mitfa:Cas9

    Plasmid

    #118840

    Purpose

    Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT01

    Plasmid

    #223373

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MS2-Tet1-CD

    Plasmid

    #83341

    Purpose

    To introduce MS2 coat protein fused Tet1-CD

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    MS2-Tet1-CD
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEP179_pX330K

    Plasmid

    #137882

    Purpose

    Entry vector for multi-gRNA cloning

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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