We narrowed to 6,268 results for: kit

1. pDONR221 Cas9-POLD3

   Plasmid

   #183197

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-POLD3
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p1977 (pUC19-PTDH3-PTEF1)

   Plasmid

   #78230

   Purpose

   Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   Bidirectional promoters pTDH3-pTEF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pSF2C-A-RFP

   Plasmid

   #172432

   Purpose

   StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NAX_CAGGS_CAS9_m4_KanR neo

   Plasmid

   #167869

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dsRedm-Rab21

   Plasmid

   #83425

   Purpose

   Full-length mouse Rab21 (wildtype)

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   dsRed-monomer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_VPR_Hygro

   Plasmid

   #167883

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138731

   Purpose

   Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB3034(X-3 MarkerFree)

    Plasmid

    #73272

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138734

    Purpose

    Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

    Plasmid

    #55199

    Purpose

    CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    ECFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJG363

    Plasmid

    #91183

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJG370

    Plasmid

    #91187

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gRNActrl+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDendra2-Hygro

    Plasmid

    #202407

    Purpose

    Dendra2 expression plasmid

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    Hygromycin
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_A0601

    Plasmid

    #91016

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBTK563

    Plasmid

    #110612

    Purpose

    BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_C2813

    Plasmid

    #91092

    Purpose

    Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi2

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK0_017

    Plasmid

    #123932

    Purpose

    Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI attB tagBFP destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBTK124

    Plasmid

    #110583

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP25 promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDN43

    Plasmid

    #61346

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS11 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMOD_B2517

    Plasmid

    #91074

    Purpose

    Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTK403

    Plasmid

    #110599

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-ING5

    Plasmid

    #13290

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING5 (ING5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pMOD_C2806

    Plasmid

    #91091

    Purpose

    Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    FMV 34S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221 Cas9-RFC5

    Plasmid

    #183200

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC5
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV-Y-GECO1

    Plasmid

    #55766

    Purpose

    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Integr Biol (Camb). 2014 May 19.

    Insert

    Y-GECO1.0
    

    Use

    Tags

    6x His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBW313lux-hrpR

    Plasmid

    #61436

    Purpose

    pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

    Depositor

    Baojun Wang, Martin Buck

    Article

    Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

    Insert

    F2620-rbs33-hrpR-ter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    plux

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NAX_CAGGS_CAS9_m4_VPR_Puro

    Plasmid

    #167887

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBTK401

    Plasmid

    #110597

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MTK3_020

    Plasmid

    #123735

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ERT2-fkbp-spdCa9(205-1368)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCC20

    Plasmid

    #198783

    Purpose

    Drives specific expression in the ASK neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Psra-9-GAL4-SK(DBD)-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMOD_A0801

    Plasmid

    #91022

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXs-ms-Sox1

    Plasmid

    #50778

    Purpose

    retroviral expression of mouse Sox1

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox1 (Sox1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTC365

    Plasmid

    #91214

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge