We narrowed to 17,082 results for: EMB

1. pcDNA3-AktAR-Kras

   Plasmid

   #123357

   Purpose

   FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR-Kras
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK520

   Plasmid

   #110604

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Tmem214 pmRFP-N2 (1348)

   Plasmid

   #62047

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem214 (TMEM214 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

4. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBTK510

   Plasmid

   #110611

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMV_AA090

   Plasmid

   #216099

   Purpose

   Knockout, EF1a-driven Cas12a (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK564

   Plasmid

   #110613

   Purpose

   BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Nanoluc
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTHMT_hnRNPF_FL

   Plasmid

   #139114

   Purpose

   express FL hnRNPF with N-terminal maltose binding protein tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPF (HNRNPF Human)
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tmem214 pEGFP-N2 (1072)

   Plasmid

   #62026

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem214 (TMEM214 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCDH-CIBN-CAAX

    Plasmid

    #188989

    Purpose

    A lentiviral plasmid encoding plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    CAAX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ macroH2A1.2-GFP-HA

    Plasmid

    #30515

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Xenopus/zebrafish expression.

    Tags

    eGFP and triple HA

    Expression

    Mammalian

    Mutation

    NO STOP

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV115

    Plasmid

    #63182

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTK509

    Plasmid

    #110610

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBTK121

    Plasmid

    #110581

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    PA3 Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. MAC_C_LMTK2

    Plasmid

    #187752

    Purpose

    MAC-tagged gene expression of human LMTK2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LMTK2 (LMTK2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-UTRN-mini

    Plasmid

    #158749

    Purpose

    Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    UTRN-mini
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC_C_IGF1R

    Plasmid

    #187770

    Purpose

    MAC-tagged gene expression of human IGF1R

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    IGF1R (IGF1R Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF(UG) hSyn PM-SYT7alpha deltaTMD

    Plasmid

    #179720

    Purpose

    Lentiviral expression of plasma membrane-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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