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pcDNA3.1(+)-GFP11-RhoA

Plasmid

#181860

Purpose

Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-RhoA (RHOA Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37C-A44C

Plasmid

#162582

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Alanine 37 changed to Cysteine and Alanine 44 cha…

Promoter

polyhedring

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_Q141V-P221L

Plasmid

#162584

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glutamine 141 changed to Valine and Proline 221 c…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_L144C-P221C

Plasmid

#162585

Purpose

Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Leucine 144 changed to Cysteine and Proline 221 c…

Promoter

polyhderin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_G131C-N172C

Plasmid

#162586

Purpose

Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glycine 131 changed to Cysteine and Asparagine 17…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N167C-L169C

Plasmid

#162587

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Asparagine 167 changed to Cysteine and Leucine 16…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1-ORF24-2xStrep

Plasmid

#138454

Purpose

Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

BcRF1 amino acids 1-178 fused to ORF24 amino acid…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-ORF24-2xStrep

Plasmid

#138455

Purpose

Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87-ORF24 (UL87, ORF24 HCMV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

UL87 amino acids 1-248 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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rd

Plasmid

#112912

Purpose

Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

Depositor

C. James McKnight

Article

Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

Insert

dematin (DMTN Human)

Tags

Glutatione-S-Tranferase and precision protease si…

Expression

Bacterial

Mutation

PEST sequence near the N-terminal removed. 5′-GCG…

Promoter

T7

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pOUPc-Rh159-GFP

Plasmid

#179280

Purpose

All in one "tet-on" lentivirus vector that expresses rhesus cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. Rh159 is an ER resident protein that binds NKG2D activating ligands.

Depositor

Jeremy Kamil

Article

Zhang et al mBio. 2019 Dec 10;10(6). pii: mBio.02110

Insert

Rh159 fused to eGFP

Use

Lentiviral; All-in-one "tet" on lentivi…

Tags

eGFP

Expression

Mammalian

Promoter

Tet Responsive Element 3G

Availability

Academic Institutions and Nonprofits only

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pOUPc-Rh159-HA

Plasmid

#179344

Purpose

All-in-on "tet-on" 3G lentiviral vector plasmid encoding rhesus cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA tag fused to its cytoplasmic tail

Depositor

Jeremy Kamil

Article

Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00

Insert

Rh159

Use

Lentiviral; All in one tet-on lentiviral vector (…

Tags

HA tag

Expression

Mammalian

Promoter

tet responsive element 3G

Availability

Academic Institutions and Nonprofits only

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Expression

Mammalian

Promoter

Oct4

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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SFFV-ETV3-Brd 283

Plasmid

#219224

Purpose

Transcription factor ETV3 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ETV3 (ETV3 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-748pUC19-tNGFR-P2A-AP3B1

Plasmid

#186106

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

AP3B1 (AP3B1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ETV3

Plasmid

#88804

Purpose

Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ETV3 (ETV3 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)

Tags

His6

Expression

Bacterial

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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