We narrowed to 17,662 results for: por
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Plasmid#107461PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCEP4-SERTA169D-myc
Plasmid#107460PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC3R112C/T180C
Plasmid#85088PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450cam
TagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3PromoterT7 promoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS-3R112C/T180C
Plasmid#85095PurposeHost vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationchanged Arginine 112 to Cysteine and Threonine 18ā¦PromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(L)Cas9
Plasmid#127385PurposeExpresses Cas9 at low levelDepositorInsertuORF(L)-Cas9
UseCRISPRExpressionInsectAvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
ExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FlaviA-GFP-puro
Plasmid#140088PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
XRE-pNL1.3[secNLuc]
Plasmid#182295PurposeSecreted Luciferase reporter for AhR activityDepositorAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochondā¦PromoterCMVAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVPromoterCustomAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorInsertmCherry codon 59 gRNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl1
Plasmid#86678PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
ExpressionMammalianAvailable SinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termiā¦PromoterCMVAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
hGAPDH-rLuc
Plasmid#82479Purposereporter of GAPDH expressionDepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD NFIL3
Plasmid#179512PurposeTunable and temporal expression control of NFIL3DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only