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mEos2-H2B-6

Plasmid

#57384

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H2B (H2BC11 Human)

Tags

mEos2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Flag YAP S381A

Plasmid

#27377

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S381A

Available since

March 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-BMPR1B

Plasmid

#23638

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR1B (BMPR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMVtight-UPRT-T2A-RFP-IRES-CD

Plasmid

#126677

Purpose

Doxycycline inducible expression of UPRT and CD along with reporter RFP

Depositor

Joan Massague

Publication

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)

Promoter

tight TRE

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHyg-AID*-GFP

Plasmid

#99521

Purpose

C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

GFP

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBa-tdTomato-flag-BicD2 594-FKBP

Plasmid

#64205

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Publication

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP, flag, and tdTomato

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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SAP130

Plasmid

#11241

Depositor

Robin Reed

Publication

Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

Insert

SAP130 (SF3B3 Human)

Expression

Bacterial

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Publication

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Mammalian

Promoter

U6

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Fibronectin-human-EDB in pMAX

Plasmid

#120403

Purpose

enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Publication

Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

Insert

EDB-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ10878

Plasmid

#183964

Purpose

CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

Depositor

Stanley Qi

Publication

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D156R, E292R, D235R, D350R

Promoter

CAG

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LP110a

Plasmid

#90199

Purpose

pEGFP-N1 with cDNA encoding full length HsKIF13A

Depositor

Lotte Pedersen

Publication

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13A (KIF13A Human)

Tags

GFP

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Insert

Pmyo-2::GFP::unc-54_3'UTR

Expression

Worm

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZac2.1 hSynapsin1 NAPA-N SV40

Plasmid

#92282

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Publication

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Promoter

hSynapsin1

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Publication

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PIP5K1A

Plasmid

#23755

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1A (PIP5K1A Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMV306DIG13+FFlucRT

Plasmid

#49998

Purpose

Integrase removed to increase stability of the plasmid in mycobacteria

Depositor

Brian Robertson

Publication

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pICE-FLAG-NAT10-siR-G641E

Plasmid

#59366

Purpose

Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Publication

Larrieu et al Science. 2014 May 2;344(6183):527-32. do

Insert

NAT10 NM_024662 (NAT10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

G641E and silent mutations to render the cDNA res…

Promoter

CMV-tet

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF149 pSOD1G85RAcGFP1

Plasmid

#26410

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G85R

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles